摘要
经菌种筛选确定为串珠镰孢AS 3,2 96 0菌株 ;对影响赤霉素产率的固态分批补料发酵的 7个因素进行了正交试验 ,从而筛选出最佳发酵条件为 :在 32℃下 ,按培养基初始pH值 4 5 ,接种量 2 0 %的比例 ,在第 3天添加13 2 %的玉米淀粉 ,从第 3天通气 ,共培养 6d ;对固态分批补料发酵与传统的液态发酵产生赤霉素能力进行了比较 ,结果表明 :其固态分批补料培养技术为液态发酵的 3～ 4 7倍 ;对发酵产物进行了分离提取效果的比较实验 ,结果表明 :以 10 %的乙醇 ,在pH2 5下提取效果最佳 ;进行了多级逆流浸提试验 ,赤霉素含量达到 0 70g/kgDMB(发酵物 )
This research decides Fusarium monilifome AS 3,2960 by screening. Six-level orthogonal tests were made with seven factors which influence on the capability of gibberellin; while the best ferment conditions were selected, which are in 32℃,the beginning pH 4 5 of culture medium ,20% of inoculating quantity, adding 13 2% corn starch in the third day, ventilating in the third day and the fermentation being 6 days. Under the same fermentation condition, the gibberellin production prepared by fed in batches solid-state and liquid-stat fermentation was compared. The results showed that the quantity of gibberellin production by fed in batches solid-state fermentation is about 3-4 3 times as much as that by liquid-state fermentation; with vary solvents and vary pH value the comparison tests of the effect of gibberellin separating-extraction showed that 10% alcohol was the best condition in pH 2 5 ; Throngh the multilevel backwash swimcarry test, the production rate of gibberellin reaches 0 70 g/kg DMB
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第3期323-328,共6页
Journal of Southwest China Normal University(Natural Science Edition)
基金
重庆市教委资助项目
关键词
固态分批补料培养
分离提取工艺
赤霉素
发酵
Fusarium moniliform AS 3,2960
the best fermentation conditions
fed in batches solid state fermentation
the effect of gibberellin separating-extraction
