摘要
提出了一种改进的SDS -PAGE方法 .采用 0 .46mol/LTris - 0 .0 47mol/Lglycine (pH 9.5 7) + 1.0g/LSDS连续缓冲体系 ,分离胶T =15 % (C =3 % )和浓缩胶T =4% (C=3% ) 时在 3.5～ 6 8.0kD分子量范围内具有良好的线性关系 ,相关系数r =0 .94.该法操作简便分离效果好 ,使用常用的电泳试剂 。
An improved SDS-PAGE method for the separation of low molecular weight polypeptides is studied in this paper. A continuous buffer of 0.46?mol/L Tris-0.047?mol/L glycine (pH?9.57)+1.0?g SDS,is used as buffer for both separation gel and stacking gel as well as electrode buffer in our method. Polypeptides with molecular weights from 3.5 to 68.0?kD can be satisfactorily separated with good linearity (correlation coefficient r=0.94) between the relative mobility and the logarithm of molecular weight. It is a SDS-PAGE method with the advantage of being the most widely used one with greater ease and lower cost, and it is very useful for the study of enzymatic cleavage products and diseases.
出处
《福州大学学报（自然科学版）》
CAS
CSCD
2001年第3期113-115,共3页
Journal of Fuzhou University(Natural Science Edition)
基金
福建省"2 11"重点学科基金资助项目 !(2 12 15 )
