摘要
目的 :研究经无唾液酸胎球蛋白 (asialofetuin ,AF)修饰后 ,脂质体达到小鼠肝实质细胞靶向的可能性。方法 :用放射性同位素标记的方法测定普通长循环脂质体 (stericallystabilizedliposomes,SSL)、AF 修饰普通脂质体(AF linkednormalliposomes,AF NL)及AF 修饰长循环脂质体 (AF linkedstericallystabilizedliposomes,AF SSL)在小鼠血、心、肝、脾、肺、肾等各器官及肝内不同细胞中的分布。结果 :SSL、AF NL及AF SSL的血中半衰期分别为14.44、4.73、11.49h ;整个肝中的分布AF NL >AF SSL >SSL ,经t化极差 q法检验 ,三者之间差异均存在显著性 ;肝不同细胞 (即实质与非实质细胞 )中脂质体的浓度均为AF NL >AF SSL >SSL(P <0 .0 5 ) ,但实质细胞与非实质细胞的浓度比为AF NL≈AF SSL SSL(P <0 .0 5 )。结论 :经AF修饰后 ,无论是否长循环 。
To study the possibility of liposomes to target hepatocytes after being linked with asialofetuin(AF). Methods: The biodistributions in different cells in liver as well as in blood, in various organs such as heart, spleen, lung and kidney of mice, of traditional sterically stabilized liposomes(SSL), AF linked normal liposomes(AF NL) and AF linked sterically stabilized liposomes(AF SSL) were studied by radioisotopic labeling. Results: The half lives of SSL, AF NL and AF SSL were 14.44, 4.73 and 11.49 h, respectively. The distribution of liposomes in liver was AF NL>AF SSL>SSL. There was significant difference among these three formulations. In hepatocytes and non hepatocytes, the concentrations of the liposomes were both in line with the sequence AF NL>AF SSL>SSL( P <0.05). However, the ratios of the concentrations in hepatocytes to that of non hepatocytes were AF NL≈AF SSLSSL( P <0.05). Conclusion: After being labeled with asialofetuin, liposomes can target hepatocytes very well, whether in long circulation or not
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2001年第3期251-254,共4页
Journal of Peking University:Health Sciences
基金
国家自然科学基金!(39770 879)资助&&
关键词
细胞靶向性
无唾液酸胎球蛋白
脂质体
代谢
肝代谢
Liver/metab
Cell targeting
Asialofetuin
Liposomes/metab
Alphafeto proteins/metab
