摘要
目的 :建立过度刺激卵巢模型 ,探讨血小板活化因子 ( PAF)在卵巢过度刺激综合症( OHSS)发病中的变化。方法 :未成年 Wistar雌鼠 ,连续 4d皮下注射 PMSG1 0 IU,d5注射 h CG 3 0 IU,48h后取腹腔灌洗液 ,用分光光度计测腹腔和卵巢的血管通透性。取尾静脉血测 PAF含量、血浆血小板活化因子乙酰水解酶 ( PAF-AH)活性及血小板聚合功能。另设生理盐水对照组。结果 :PMSG+h CG组腹腔灌洗液中美兰的含量为 5.6 1 8±1 .1 3 3μg/1 0 0 g体重 ,显著高于对照组的 0 .976± 0 .2 2μg/1 0 0 g体重 ( P<0 .0 1 )。卵巢PAF含量 PMSG+h CG组为 1 1 .3 51± 0 .46 9pmol/卵巢 ,显著高于对照组的 1 .0 0 0±0 .1 0 8pmol/卵巢 ( P<0 .0 1 )。PAF-AH活性 :PMSG+h CG组 d5和 d7分别为 40 .0 2 7±1 3 .791 pmol/min· ml和 1 8.90 9± 9.783 pmol/min· ml,显著低于对照组的 57.875±1 4 .2 2 8pmol/min· ml和 53 .82 1± 1 6 .71 4 pmol/min· ml( P<0 .0 1和 P<0 .0 0 1 )。结论 :注射 PMSG和 h CG后 ,雌鼠卵巢 PAF含量增高 ,并使腹腔和卵巢血管通透性增高 ,导致 OHSS。血浆 PAF-AH在 OHSS发病中可能通过调节 PAF的水平而发挥作用。
WT5HZ] Objective:To explore the relationship between the platelet activating factor and the pathogenesis of ovarian hyperstimulation syndrome (OHSS). Methods:Immature female Wistar rats were divided into two groups: the vehicle group (38 rats ) and the PMSG + hCG group (36 rats). Ovarian hyperstimulation model was set up among immature female Wistar rats. Rats were injected via the tail vein evens blue dye (EB) solution to evaluate the capillary permeability in the peritoneal cavity. Blood samples were collected from the tail vein. Results:The EB level of the peritoneal cavity in the PMSG+ hCG group was significantly higher than that in the vehicle group. The content of platelet activating factor (PAF) in the ovary in the PMSG + hCG group was markedly higher than that in the vehicle group. Plasma platelet activating factor acetylhydrolase (PAF AH) activity in the PMSG + hCG group was significantly decreased compared with that in the vehicle group on day 5 and on day 7.Conclusion:Pathogenesis of OHSS was probably induced by the increase of PAF and the enhancing of capillary permeability after the injection of PMSG and hCG.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2001年第3期161-165,共5页
Reproduction and Contraception
关键词
血小板活化因子
血不上板活化因子乙酰水解酶
卵巢过度刺激综合症
platelet activating factor(PAF), platelet activating factor acetylhydrolase (PAF AH), ovarian hyperstimulation syndrome(OHSS)
