摘要
目的观察长期注射大剂量同种异体抗角蛋白自身抗体(AK auto Ab)后，家兔血清中针对抗角蛋白自身抗体(AK auto Ab)独特型抗体的产生。方法肌肉注射亲和层析纯化的AK auto Ab(5 mg/kg)，隔日1次，连续90 d，用ELISA检测血清中的抗独特型抗体。结果注射AK auto Ab的家兔产生了针对AK auto Ab F(ab′)2片段的抗独特型抗体，于给药4 wk血清抗体滴度达高峰，以后逐渐降低。结论长期注射大剂量同种异体AK auto Ab的家兔可产生免疫耐受性。
Aim To observe the production of anti-idiotypic antibodies directed against AK auto Ab in the rabbit sera after long-term, high dose allogenic AK auto Ab injection. Methods Allogenic AK auto Ab (5mg/kg)was injected intramuscularly to rabbit once every other day for 90 days. Anti-idiotypic antibodies in rabbit sera were detected by ELISA. Results Rabbits injected with AK auto Ab generated anti-idiotypic antibodies that could react to F(ab′)2 of AK auto Ab. The titers of anti-idiotypic antibodies reached the highest levels at 4 weeks after the administration of AK auto Ab and then gradually decreased. Conclusion Rabbits could be induced to form immune tolerance when high-does allogenic AK auto Ab is administrated for long-term.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2001年第4期371-373,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家1035课题资助
No. 96-901-01-38
