摘要
目的 建立口腔癌原代培养细胞 MTT药敏试验的方法。方法 采用 MTT法对 2 8例口腔癌新鲜组织和 TCa8113系、ACC- 2细胞株进行药物敏感性检测 ,比较分析各操作步骤对检测结果的影响。结果 MTT法能准确检测口腔癌肿瘤细胞系 (株 )和新鲜口腔癌组织的药物敏感性。但在后者的检测中应特别注意肿瘤组织的取材、肿瘤细胞悬液的制备、肿瘤细胞的培养、MTT的温育时间以及甲浮液的离心等操作过程。结论 MTT法能准确检测新鲜口腔癌组织的药物敏感性 ,且重复性好 ,检测周期较短 ,适合大批量样本或大批量药物的筛选 ,同时无放射性污染。
Objective To establish chemosensitivity testing of oral cancer using the MTT assay. Methods MTT assay was performed for chemosensitivity testing of 28 cases of oral cancer and TCa8113, ACC 2 cell lines. The effect of experiment processes on results of trial was analyzed comparatively. Results MTT assay could examine precisely chemosensitivity of oral tumor cell lines and fresh tumor tissue. But these processes of chemosensitivity testing of fresh oral cancer tissue using the MTT assay were noticed specially, such as selecting tumor tissue, preparing tumor cell suspension, culture of tumor cell, incubation time of MTT and centrifuge of formazan. Conclusion Chemosensitivity of fresh oral cancer tissue could be examined precisely using the MTT assay. This chemosensitivity testing using MTT assay had many advantages, such as better repetition, shorter examination periods and choosing of great number of drugs. In this trial processes there was no radioactive pollution.
出处
《口腔颌面外科杂志》
CAS
2001年第2期132-134,共3页
Journal of Oral and Maxillofacial Surgery
关键词
口腔癌
细胞培养
MTT法
药敏试验
Oral cancer
Cell culture
MTT assay
Chemosensitivity test
