摘要
目的 研究ＨＩＶ－１相关等位基因（CCR5、CCR2b和SDF1在性病、艾滋病感染人群中的分布，为分析这些宿主遗传因素在ＨＩＶ－1感染、艾滋病发病进程中的作用提供理论依据。方法 收集性病、艾滋病感染者的血液标本，提取基因组ＤＮＡ，经PCＲ或ＰＣＲ-ＲＦＬＰ分析，计算突变基因频率。结果ＣＣR2ｂ-６４Ｉ和ＳＤＦＩ－３'Ａ的突变频率较高。２１５例性病患者和７５例艾滋病感染者ＣＣＲ2ｂ-６４Ｉ的突变频率分别为１８．６０％和１５．３３％，ＳＤＦＩ－３'Ａ的突变频率分别为２４.４２％和２３．３３％。但ＣＣＲ５△３２的突变频率较低（０．２３％），而 ＣCR5m303等位基因突变率为0,这与中国普通汉族人和该地区普通汉族人的结果一致。结论 提示在遗传机制上艾滋病对中国人构成的威胁比西方人更大。
Objective To study the distribution of CCR△532, CCR5m3O3, CCR2b-64 I and SDF1-3 'A alleles in the patients with STD and HIV- 1 carriers, and to evaluated the role of these alleles in the development of the diseases. Methods The genomic DNA from 290 patients with STD(215) and HIV-1 carriers (75) in Henan province of China were purified from whole blood samples by QIAmp DNA Blood Mini Kit and identified by means of PCR or PCR-RFLP analyses. Results The study showed that the mutation frequency of CCR2b-64I alleles was 18.60% and 15.33% respectively in patients with STD and HIV-1carriers, SDF1-3 'A was 24.42% and 23. 33% . The results are similar to that in Chinese normal population,which possess a characteraztion of very lower frequency of CCR5△32and CCR5m3O3,and higher frequency of CCR2b-64I and SDF1-3' A alleles.Conclusion The results suggested Chinese patients with STD may be highly susceptive to HIV-l infection, and the significances of above mutant HIV-1-related alleles need to be evaluated in HIV-1 carriers.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2001年第4期259-261,共3页
The Chinese Journal of Dermatovenereology
基金
国家自然科学基金资助(397706830)
关键词
HIV-1
遗传易感性
基因多态性
协同受体
性传播疾病
HIV-1
Genetic susceptibility
Allelic polymorphism
Coreceptor
Sexually-transmitted disease
