摘要
用离子排斥色谱法实现了对果汁中 11种有机酸 (草酸、柠檬酸、酒石酸、苹果酸、抗坏血酸、乳酸、琥珀酸、甲酸、乙酸、戊二酸、富马酸 )的分离测定。以 17mmol/L硫酸为淋洗液 ,样品在ICE ION 30 0离子排斥柱上分离后 ,用紫外检测器在 2 10nm处测定其中的有机酸。各组分质量浓度测定的相对标准偏差在 1.5 %～ 9.8% (n =10 )。
A method for the quantitative determination of 11 organic acids (oxalic acid, citric acid, tartaric acid, malic acid, ascorbic acid, lactic acid, succinic acid, formic acid, acetic acid, glutaric acid and fumaric acid) in fruit juice was developed successfully. It was based on an ion exclusion chromatographic separation under the conditions of isocratic elution with 17 mmol/L sulphuric acid solution with the ICE ION 300 ion exclusion column, and the UV detection at 210 nm. The precision of the method was investigated and the relative standard deviations were from 1.5% to 9.8%( n =10).
出处
《色谱》
CAS
CSCD
北大核心
2001年第3期276-278,共3页
Chinese Journal of Chromatography
基金
上海检验检疫局1997年科研项目
关键词
离子排斥色谱
有机酸
果汁
测定
饮料
ion exclusion chromatography
organic acid
fruit juice
