摘要
采用反相高效液相色谱法 ,在C18柱上以V(甲醇 )∶V(水 ) =2 5∶75的溶液为流动相 (内含 0 .0 5mol/L磷酸二氢钠 ) ,检测波长为 2 0 5nm ,同时分离测定诺诺感冒片中扑尔敏、扑热息痛、盐酸伪麻黄碱的含量。扑尔敏、扑热息痛和盐酸伪麻黄碱的检出限分别为 1.16mg/L ,0 .15mg/L和 1.82mg/L ,其相应的回收率分别为 98.35 % (n =5 ,RSD =1.6 0 % ) ,10 1.16 % (n =5 ,RSD =1.5 0 % )和 98.5 0 % (n =5 ,RSD =1.5 9% )。方法简便、快速 ,重现性好 ,适用于诺诺感冒片的质量检验分析。
An HPLC method is described for the determination of chlorpheniramine, paracetamol and pseudo ephedrine hydrochloride simultaneously in Nuonuo composite tablets. Chromatographic conditions were: column,YWG C 18 column (150 mm×4.0 mm i.d.,10 μm);mobile phase,25% methanol(0.05 mol/L sodium dihydrogen phosphate); flow rate,1.0 mL/min;detection wavelength,205 nm; injection volume,20 μL. The detection limits of chlorpheniramine, paracetamol and pseudo ephedrine hydrochloride were 1.16 mg/L, 0.15 mg/L and 1.82 mg/L respectively and the corresponding average recoveries were 98.35% (n =5, RSD= 1.60%), 101.16% (n =5, RSD=1.50%) and 98.50% (n =5, RSD=1.59%) respectively . The proposed method is simple, rapid and accurate and has been satisfactorily used for the quantitative analysis of real samples.
出处
《色谱》
CAS
CSCD
北大核心
2001年第3期236-238,共3页
Chinese Journal of Chromatography
基金
福州大学科技育苗基金!资助项目 (XKJ(YM 0 0 0 5 ) )
关键词
高效液相色谱法
扑尔敏
扑热息痛
盐酸伪麻黄碱
诺诺感冒片
药物分析
high performance liquid chromatography
chlorpheniramine
paracetamol
pseudo ephedrine hydrochloride
Nuonuo composite tablets
