摘要
目的 建立HPLC测定氨咖麻敏胶囊中对乙酰氨基酚、咖啡因、盐酸伪麻黄碱、马来酸氯苯那敏含量的方法。方法 采用ZORBA×SB-C18柱,以甲醇-0.05 mol·L-1磷酸二氢钾溶液-三乙胺(15:85:0.02)为流动相,同时分离测定了上述4组分的含量,进行了系统适用性、回收率和精密度等方法学研究。结果 对乙酰氨基酚、咖啡因、盐酸伪麻黄碱、马来酸氯苯那敏线性范围分别为96.7-386.7,12.9-51.5,12.8-61.4,1.35-5.41 μg·mL-1,回收率分别为:100.4％,101.3％,101.0％,101.6％,RSD分别为0.33％,0.54％,0.74％,0.73％(n=9)。结论 该法准确、简便、快速,适于氨咖麻敏胶囊的含量测定。
OBJECTIVE: To develop a HPLC method for the determinations of paracetamol, caffine, pseudepherine hydrochloride and chlorphenamine maleate respectively in their compound capsules. METHODS: ZORBA × SB-C18 column was used and the mobile phase was methanol-0.05mol·L-1 potassium dihydrogen phosphate-triacetamide (15:85:0.02). The system suitability test, recovery test and repeatability test were carried out to evaluate the applicability of the method. RESULTS: The calibration curves for paracetamol, caffine, pseudepherine hydrochloride and chlorphenamine maleate were linear over the range of 96.7 - 386.7, 12.9 - 51.5, 12.8 - 61.4 and 1.35 - 5.41 μg· mL-1, respectively. The recoveries were 0.33%, 0.54%, 0.74% and 0.73% (n=9), respectively. CONCLUSION: The method was accurate, rapid and simple, being applicable for the assay of paracetamol, caffine, pseudepherine hydrochloride and chlorphenamine maleate in their compound capsules.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2004年第7期542-544,共3页
Chinese Pharmaceutical Journal
