摘要
以温度休克方法研究了养殖大黄鱼 (Pseudosciaena crocea)三倍体诱导的处理条件 .结果表明 :1)采用冷体克和热休克方法均可诱导出三倍体大黄鱼 ,三倍体诱导率明显受处理温度、处理起始时间以及处理持续时间等因子的影响 ;2 ) 4℃冷休克条件下 ,处理起始时间为受精后 2 min,处理持续时间为 10 min有较高的孵化率和三倍体诱导率 ;3) 37℃热休克条件下 ,处理起始时间为受精后2 .5 min,处理持续时间为 5
Studies on induction of triploidy in the large yellow croaker P. crocea cultured in net cages were respectively carried out from April to September in 2000 in Zhangpu and Ning de. The fertilized eggs were treated with temperature shock at 2~6 ℃ and 37~40 ℃ for 1.5~5 min after fertilization, and the continuation of inducing triploidy was lasted for 5~20 min. The results showed that: when triploidy was induced for 2.5 min after fertilization at 37 ℃ for 5 min, the rate of occurrence of triploid was high; and those for 2 min after fertilization at 4 ℃ for 10 min, the rate of occurrence of triploid was even higher. The relationship between the rate of occurrence of triploid and the survival rate were also discussed.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第4期927-930,共4页
Journal of Xiamen University：Natural Science
基金
国家高技术研究发展 86 3计划资助项目 (86 3- 819- 0 2 - 12 )
