摘要
目的 :研究人发角蛋白 (HHK)在脊髓损伤 (SCI)修复中诱导和促进神经元和神经纤维的再生以及HHK在脊髓中的降解机理。方法 :选用 1 2只成年雌性SD大鼠 ,采用改制的Ⅱ型NYU装置 ,建立SCI组、SCI后植入HHK组 ,并设正常对照组。分别于术后 1 4d取材 ,经HE、Mallory’s 磷钨酸 苏木素、Loyer’s SterryThionin等方法染色 ,观察其光镜结构的变化。结果 :与损伤组相比较 ,植入HHK组损伤节段的萎缩程度明显减低 ,灰质中部分神经元残存 ,有髓神经纤维脱髓鞘现象减轻 ;HHK周边集聚大量巨噬细胞和胶质细胞 ;植入的HHK毛小皮膨胀松弛 ,出现与皮质层分离 ,皮质层内出现皲裂 ,中央髓质腔增大。结论 :植入HHK具有减轻脊髓损伤后的继发性损伤的作用 ,为进一步研究HHK在SCI修复过程中的作用及机理提供了形态学基础。
Objective: To study the histological changes of transplanted human hair keratin(HHK) to repair acute spinal cord injury (SCI) in adult rat.Methods: A 10g rod was dropped over a distance of 25 mm onto the exposed T 10 cord of rats using the modified weight drop device from New York University (NYU). The HHK was transplanted into the SCI rats subsequently.These samples were stained with Hematoxylin Eosin, Mallory's Phosphotungstic acid Hematoxylin, Loyer's, and Sterry's Thionin at week 2, and the histological changes of the samples of the SCI repair were observed by optical microscopy.Results: Shrink degree of the spinal cord transection area in the transplanted HHK group was lower than that of no transplant group.In the transplanted HHK group, partial neurons of gray matter remained, demyelination and microcysts phenomenon decreased.Macrophages and oligodendrocytes assembled around the HHK.Hair cuticle became loose.The enter cavity of hair medulla was enlarged.Conclusions:HHK can intervent with the pathological processes and promote the function recovery of spinal cord.The HHK can boost the regeneration of glial cell and reduce myelinoclasis.The study provide morphological basis for finding the repair principle HHK in SCI.
出处
《中国临床解剖学杂志》
CSCD
北大核心
2001年第3期243-244,共2页
Chinese Journal of Clinical Anatomy
