摘要
目的 探讨不同病程糖尿病大鼠血浆肿瘤坏死因子－α（ＴＮＦ－α）水平与糖尿病大鼠光、电镜下视网膜病理改变及病情相关指标的关系。方法 复制糖尿病视网膜病变（ＤＲ）大鼠模型，分别在其糖尿病持续３～６个月时，对其视网膜进行形态学观察，采用酶联免疫吸附法测定血浆ＴＮＦ－α水平，同时测定血糖（ＢＧ）、糖化血红蛋白（ＧＨｂＡ１Ｃ）、血浆胰岛素（Ｉｎｓ）及Ｃ肽（ＣＰ）水平。结果 相同病程模型组与治疗相比较，ＴＮＦ－α、ＢＧ、ＧＨｂＡ１ｃ显著升高（Ｐ＜０．０１），Ｉｎｓ、ＣＰ显著降低（Ｐ＜０．０１），病理形态学改变明显加重；不同病程间模型组比较，ＴＮＦ－α、ＢＧ、ＧＨｂＡ１ｃ显著升高（Ｐ＜０．０１），Ｉｎｓ、ＣＰ显著降低（Ｐ＜０．０１），病理形态学改变明显加重，可见新生血管；不同病程治疗组间比较，ＴＮＦ－α、ＢＧ、ＧＨｂＡ１ｃ、Ｉｎｓ、 ＣＰ比较无显著差异，６个月组大鼠视网膜病理改变较３个月组无减轻。 结论 ＴＮＦ－α水平随病情加重、病程延长、视网膜病变加重而增高，ＴＮＦ－α可能是ＤＲ的一个重要危险因素，参与了ＤＲ的病理发展过程。
Objective To study the relationship between serum level of TNF-α and morphologic changes as well as related factors in 3 and 6 months of diabetic retinopathy rats. Methods This research successfully duplicated a model of insulin dependent diabetes mellitus in rats and carried out morphologic observation of retina of diabetic rat when the disease lasted for 3 and 6 months. The serum level of TNF-α by ELISA together with related factors, such as blood glucose (BG)by oxidase method、glucosylated hemoglobin (GHbAlc) 、serum insulin (Ins) and c-peptide (C-P) by RIA were studied. Results In same duration,the levels of TNF-α、BG、GHbAlC in model groups were significantly higher than therapy groups (P<0.01), the levels of Ins and C-P were significantly lower. Morphologic changes were clear. In different duration of model groups,the level of TNF-α in 6 moths groups was significantly higher than that of 3 months. Morphologic changes were clear in 6 months. There was no different in serum TNF-α、BG、GHbAlC、Ins、C-P between different duration of therapy groups. Conclusion The serum level of TNF-α was related with morphologic changes of retina、poor blood glucose、duration. The high level of TNF-α was a risk factor for the development of DR. It maybe an index to monitor the progression of DR.
出处
《中国糖尿病杂志》
CAS
CSCD
2001年第3期139-142,共4页
Chinese Journal of Diabetes
基金
黑龙江省科学技术委员会基金资助项目(97Q10)
