摘要
目的 了解肿瘤抑制基因p5 3在肝细胞癌中的突变情况 ,探讨乙型肝炎病毒 (HBV)感染与p5 3基因突变之间的关系。方法 提取 5 0例有乙型肝炎病毒感染史肝癌患者手术样本中的DNA ,用聚合酶链式反应 (PCR)扩增 5～ 9外显子 ,作单链构象多态性 (SSCP)分析。结果 p5 3基因突变率超过 2 6 % ,突变主要分布于 5～ 8外显子 ,5、6、7、8外显子分别有 3、3、4、3例 ,另有 4个可疑突变。结论p5 3基因突变可能是肝细胞癌的病因之一 ,而乙型肝炎病毒感染在中国肝癌患者p5 3基因突变中可能起到比较重要的作用。
Objective To study p53 gene mutation in human hepatocellular carcinoma (HCC) samples and the relationship between p53 gene mutation and hepatitis B virus (HBV) infection. Methods DNA samples were prepared from 50 specimens of HCC patients that had been infected with HBV. Exons 5～9 were amplified with polymerase chain reaction (PCR), and then detected by single strand conformation polymorphism (SSCP). Results Over 26% (13/50) of p53 DNA samples were found to be mutated, mainly in exons 5～8 relating to 3,3,4,3 cases respectively and along with 4 suspicious samples. Conclusion p53 gene mutation might be one of the causes of HCC, and HBV infection may be associated with such mutation in China.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2001年第2期163-165,共3页
Chinese Journal of Experimental and Clinical Virology
