摘要
将人纤维蛋白凝胶作为小鼠囊胚体外三维培养的支持物 ,建立小鼠囊胚体外三维植入模型 ,用以观察囊胚在体外的立体生长情况 .通过实验表明人纤维蛋白凝胶对培养小鼠囊胚的孵出率、扩展率均无影响 .在人纤维蛋白凝胶上培养的囊胚可以保持囊胚的立体结构 ,并有滋养层细胞极性的迁移和部分细胞的植入 ,使其更接近于体内囊胚的着床行为 .说明人纤维蛋白凝胶可以作为小鼠囊胚三维培养的支持物 ,为研究小鼠囊胚植入机制建立实验基础 .
Mouse blastocyst was cultured on human fibrin gel to develop a new three-dimensional embryo invasion model。 Human fibrin gel was found with no influence on the culture of mouse blastocyst . The whole blastocyst retained three-dimensional structure, trophoblast did not flatten but retained a polarized removal and invasion like in vivo . It suggested human fibrin gel could be used as substratum of in vitro three-dimensional model for investigating blastocyst invasive behavior.
出处
《生命科学研究》
CAS
CSCD
2001年第2期169-172,共4页
Life Science Research
基金
"九五"国家重点科技攻关项目!资助 (96 -90 5 -0 2 -0 1)
