摘要
目的 探讨白细胞介素 18(IL 18)在乙型肝炎病毒 (HBV)感染中的作用。方法 从 5 7例HBV感染者 (无症状携带者 15例 ,慢性肝炎患者 30例 ,重型肝炎患者 12例 )和 10例健康献血员血液中分离出外周血单个核细胞 (PBMC) ,在脂多糖 (LPS)刺激 6h后 ,应用逆转录聚合酶链反应 (RT PCR)及流式细胞术技术对IL 18基因的转录及表达水平进行检测。结果 不同临床类型HBV感染者之间IL 18基因转录及表达水平的差异均有非常显著意义 (P <0 .0 1) ,重型肝炎组IL 18基因转录及表达水平高于正常对照 (均P <0 .0 1) ,无症状携带者组低于正常对照组 (P <0 .0 5 ,P <0 .0 1) ,慢性肝炎组与正常对照组比较 ,差异无显著意义 (均P >0 .0 5 )。随着病情加重 ,IL 18水平上升。肝组织炎症活动度不同的慢性肝炎患者之间IL 18基因转录及表达水平的差异均有显著意义 (P <0 .0 5 ) ,肝组织炎症程度越重 ,IL 18水平越高。慢性肝炎组患者IL 18基因的转录及表达水平均与血清丙氨酸转氨酶含量呈明显正相关 (r=0 .5 4,P <0 .0 1;r=0 .6 3 ,P <0 .0 1)。无症状携带者、慢性肝炎患者、重型肝炎患者PBMC内IL 18mRNA表达水平与PBMC内IL 18蛋白含量呈高度直线正相关 (r=0 .980 ,P <0 .0 0 1;r=0 910 ,P <0 .0 0 1;r=0 .975 ,P <0 .0 0 1)。结论?
Objective To evaluate the possible roles of interleukin 18 (IL 18) in hepatitis B virus (HBV) infection. Methods Peripheral blood lymphocytes from 3 groups of HBV infectors (including 15 asymptomatic carriers,30 patients with chronic hepatitis, 12 patients with fulminant hepatitis) and 10 blood donors( as normal controls) were analyzed. The transcription and expression of IL 18 in PBMC were assessed by semi quantitive reverse transcription polymerase chain reaction and flow cytometry. Results (1) There were significant differences in the transcription and expression levels of IL 18 between each group of HBV infectors. Interestingly, it was highest in fulminant hepatitis group, and lowest in asymptomatic carrier group. However there was no significant difference between chronic hepatitis and normal controls. The severity of disease was accompanied with the elevation of IL 18 production. (2) The severity degree of liver inflammation in chronic hepatitis was ccompanied with the elevation of IL 18 production. (3) The transcription and expression of IL 18 in chronic hepatitis B correlated with serum ALT positively( r =0.54, P <0.01; r =0.63, P <0.01). (4) There was a positive correlation in transcription and expression of IL 18 in each group of HBV infectors (asymptomatic carrier r =0.980, P <0.001; chronic hepatitis r =0.910, P <0.001; fulminant hepatitis r =0.975, P <0.001). Conclusion IL 18 may take part in the host immunity against HBV,and relate to the inflammation degree of liver.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2001年第11期655-658,共4页
National Medical Journal of China
