摘要
目的 :探讨原发性肝癌患者庚型肝炎病毒 (HGV)感染的状态。方法 :根据中国株 HGV 5 '端非编码序列设计引物 ,采用逆转录套式聚合酶链反应方法。用此技术对 10 7例原发性肝癌、45例慢性肝炎、43例肝硬化患者血清进行了检测分析。结果 :10 7例原发性肝癌患者中有 10例 HGV- RNA阳性 (9.3% ) ;45例慢性肝炎患者中有 5例HGV- RNA阳性 (11.1% ) ;43例肝硬化患者中有 3例 HGV- RNA阳性 (7.0 % )。结论 :庚型肝炎病毒感染可能与原发性肝癌发生有一定关系 。
Objective:To investigate the prevlence of hepatitis G virus (HGV) infection in the cases with primary Hepat- ocellular carcinoma (PHC) .Methods: Two pairs of primers were designed based on the sequence of the 5'non-coded region of Chinese HGV isolated and HGV-RNA was detected with RT-PCR. Serum specimens 107 cases with PHC,45 cases with chronic hepatitis and 43 cases with liver cirrhosis were tested. Results: HGV was positive in 10(9.3%)out of 107 cases with PHC; 5(11.1%) out of 45 cases with chronic hepatitis; 3(7.0%) out of 43 cases with liver cirrhosis.Conclusion: There may be some relationship between HGVinfection and PHC,but HGV infectionis may be not the main cause of PHC.
出处
《南通医学院学报》
2001年第2期134-134,共1页
ACTA Academiae Medicinae Nantong
关键词
庚型肝炎病毒
原发性肝癌
逆转录套式聚合酶链反应
Hepatitis G virus
Primary hepatocellular carcinoma
Reverse transcription nested polymerase chain reaction
