摘要
目的观察兔颈动脉内支架置入后,血管平滑肌细胞(VSMC)的增殖及c-myc原癌基因的表达。方法将国产铂-铱合金支架置入16只家兔颈动脉,术后7、14、30和90d处死动物,行苏木素伊红(HE)和Masson三色染色、c-myc蛋白免疫组化染色及原位杂交。用透射电镜观察术后14d,VSMC超微结构的变化。结果术后7d,中膜VSMC由收缩型变为合成型,内弹力板消失,偶见炎性细胞浸润,1例血管腔内出现不完全血栓形成。术后14d,新生内膜增生明显,主要由合成型及过渡型VSMC组成。术后30d,新生内膜增生更加明显,主要为收缩型和少量过渡型VSMC及细胞外基质组成。术后90d,内弹力板恢复为弯曲的连续状,其上附着多层内皮细胞,VSMC为收缩型。术后14d透射电镜显示,合成型VSMC呈圆形或椭圆形,粗面内质网及线粒体非常丰富。支架置入后,兔颈动脉新生内膜中c-myc蛋白免疫组化及原位杂交均呈阳性,正常对照血管呈阴性。结论兔颈动脉内置入支架后,可引起VSMC中c-myc基因表达增强,其与VSMC的增殖密切相关,在支架内再狭窄的形成中发挥重要作用。
Aim To investigate the proliferation of smooth muscle cells(VSMCs) and the expression of c myc gene in rabbits carotid arteries after stenting. Methods Platinium Iridium stent were implanted into the right carotid arteries of 16 rabbits under vision. 7,14,30 and 90 days after the stenting procedure,morphological changes of VSMCs were observed under the light and transmission electron microscope. The c myc gene expression was detected by in situ hybridization(ISH)and immunohistochemical staining. Results 7 days after stenting,the type of VSMCs changed from contractile to synthetic type, there were a number of proliferative VSMCs in the neogenetic intima. On day 14 and 30, there were synthetic and transitive VSMCs. On day 90, the type of VSMCs recovered to contractile type. The ultrastructure of typical synthetic type of VSMCs were round, containing a large amount of rough endoplasmic reticulum and mitochondria. c myc gene expression were positive both by ISH and immunohistochemical staining. Conclusion c myc gene expression is increased and relate closely to VSMC proliferation after stenting. It may play an important role in the in stent restenosis.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2001年第3期228-230,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
陕西省科技研究发展计划项目
No.99K13-G8
