摘要
为了系统地研究不同光照强度下湿度以金钗石斛（Dendrobium nobile)生长的影响，在金钗石斛分蘖期，于80μmol.m^-2.s^-1、160μmol.m^-2.s^-1、320μmol.m^-2.s^-1、640μmol.m^-2.s^-1的不同光强下，各设置5个温度（15℃、20℃、30℃、35℃）梯度对石斛进行处理。结果表明：石斛的生长与代谢随温度由低到高，表现出弱－强－弱的变化规律℃；80μmol.m^-2.s^-1与640μmol.m^-2.s^-1的中、强光照下，25℃处理石斛的生长优势尤为明显；不同光强下，石斛鲜重的大多以25℃处理更快，繁殖力则以20℃和25℃处理较高；各光强下的MDA含量随温度升高而先降后升，且均以25℃最低；可溶性蛋白质、可溶性总糖及叶绿互含量则表现出随温度由低到高而先增后减的趋势，其含量最高点均出现在25℃左右；净光合速率和叶绿素含量随光强和温度的变化趋势基本一致；各种光强下的暗呼吸速率均随温度升高而增大。因此，在不同的光照条件下，石斛生长的适宜温度均在25℃左右。光温处理引起石斛生理生化过程明显的相应变化表现出：高温和弱光照条件有利于石斛的株高增长，但不利于产量和质量提高；石斛的生长与MDA含量呈显著负相关（r80=-0.9082、r160=0.8892、r320=-0.8075、r640=-0.8586)，与可溶性糖含量一定正相关（r80=-0.7673、r160=0.8892、r320=0.8179、r640=0.9278），并且石斛的生长与可溶性蛋白含量、叶绿素含量、光合速率之间的变化趋势基本一致。
The effect of varying temperature on the growth of Dendrobium nobile Lindl.was studied under different light intensities.In the tillering stage, Dendrobium nobile was treated with different temperatures (15 °C,20 °C,25 °C,30 °C and 35 °C) in different light intensities (80 μmol·m -2 ·s -1 ,160 μmol·m -2 ·s -1 ,320 μmol·m -2 ·s -1 and 640 μmol·m -2 ·s -1 ).Growth and metabolism of D.nobile peaked at 25 30 °C in 80 μmol·m -2 ·s -1 and 20 25 °C in 160 μmol·m -2 ·s -1 light intensity.At 320 μmol·m -2 ·s -1 and 640 μmol·m -2 ·s -1 of light, D.nobile growth was highest at 25 °C.At all light intensities,temperatures of 20 °C and 25 °C had a significant positive effect on reproductive capacity,while fresh weight production was strongest at 25 °C.The MDA content initially declined with increasing temperature,but increased again with the higher temperatures.The reverse trend was observed in chlorophyll,soluble protein and total sugar content.The MDA content was lowest and chlorophyll,soluble protein and total sugar content was highest in the 25 °C treatment.Net photosynthetic rate followed the same patterns as that of chlorophyll content.However,dark respiratory rate was improved with increasing temperature under each of the light intensities.These observed patterns of physiological and biochemical changes in D.nobile indicate that while higher temperatures and lower light conditions resulted in an increase in the plant height,the fresh weight and growth mass was significantly negatively correlated to the content of MDA ( r 80 = -0.908, r 160 =-0.982, r 320 =-0.808, r 640 =-0.859) but positively related to the content of total sugar( r 80 = 0.767, r 160 = 0.889, r 320 =0.818, r 640 =0.928);the growth mass showed corresponding changes with the soluble protein and chlorophyll content and photosynthetic rate.Optimal temperature for D.nobile growth is approximately 25 °C across the varied light intensities.
出处
《植物生态学报》
CAS
CSCD
北大核心
2001年第3期325-330,共6页
Chinese Journal of Plant Ecology
基金
国家自然科学基金!资助项目 ( 3 9770 90 3 )
