摘要
A procedure has been developed which allows high frequency regeneration of adventitious shoot from hypocotyl explants of Antirrhinum majus. Rapid and prolific shoot regeneration and very viable and yellowish callus formation occurred on a MS medium supplemented with 2 mg/L 6-benzylaminopurine(BAP) and 0.5 mg/L β-naphthenacetic(NAA). It was found that BAP was essential to adventitious shoot regeneration from hypocotyl explants. Both low(0.1 mg/L) and high(6 mg/L) concentration of BAP in MS medium were able to stimulate shoot development. Optimal concentration of BAP for shoot development was 2-3 mg/L. 2,4-D alone could stimulate formation of healthy and yellowish callus which, however, did not differentiate to generate shoot on BAP (0.1-6 mg/L) or BAP(2 mg/L) and NAA(0.5 mg/L) media. NAA alone could stimulate root development at the ends of hypocotyl explants. Addition of silver nitrate(AgNO3) to the medium inhibited shoot regeneration,and made all explants brown and die.Histological analysis indicated that adventitious shoot regeneration originated from epidermic cells of hypocotyl.Regenerated shoots rooted readily in MS medium without any growth regulators.
本文发展的技术可以使金鱼草下胚轴外植体不定芽高频再生。添加有２ｍｇ／ｌＢＡＰ和０．５ｍｇ／ｌＮＡＡ的ＭＳ培养基上再生芽出得又快又多，淡黄色愈伤组织易于形成并存活。但主要依赖的是６－ＢＡＰ。ＭＳ培养基中含有０．１ｍｇ／ｌ或６ｍｇ／ｌ浓度的６－ＢＡＰ均能刺激芽的发育，最适浓度为２－３ｍｇ／ｌ．仅加２，４－Ｄ，也能形成正常的黄色愈伤组织。但这样形成的愈伤组织在只含有０．１－６ｍｇ／ｌ６－ＢＡＰ或含有２ｍｇ／ｌ６－ＢＡＰ和０．５ｍｇ／ｌＮＡＡ的ＭＳ培养基上均不能分化生出再生芽来。仅加ＮＡＡ，能够刺激下胚轴形成根。加入ＡｇＮＯ＿３，则抑制不定芽的再生，并导致整个外植体变成棕色而死亡。组织化学分析结果表明：不定芽的再生源于下胚轴外植体表皮细胞。在不含有任何生长调节剂的ＭＳ培养基上，再生苗均能形成根。再生植株移栽至花盆可开花结实。
