摘要
目的:以紫花羊耳蒜无菌带芽块茎为外植体,建立其高频再生体系。方法:采用不同培养基、不同配比植物激素及不同移栽基质等,对紫花羊耳蒜进行丛生芽诱导、壮苗生根及炼苗移栽研究。结果:芽增殖的最适合培养基为1/2MS(大量元素减半)+6-BA 3.5 mg/L+NAA 0.2 mg/L,增殖系数平均达到3.24倍;适合紫花羊耳蒜离体培养壮苗生根的培养基配方为1/2MS+6-BA 0.6 mg/L+NAA 0.5 mg/L+20%的马铃薯汁,生根率可达95%;紫花羊耳蒜组培苗长出3条根时可炼苗,移栽基质以菜园土∶兰花土=1∶1为宜,移栽成活率为63.64%。结论:以紫花羊耳蒜无菌带芽块茎为外植体,能够实现其高频再生体系建立,为其人工扩大栽培奠定了良好的基础。
Objective： To establish the rapid propagation system of Liparis nigra Seidenf. by the aseptic tubers with buds. Methods： Different media, plant growth regulators at different concentration and different material were used to L.nigra Seidenf. for cluster buds induction and multiplication, rooting and transplanting. Results： The medi- um 1/2MS＋6-BA 3.5 mg/L＋NAA 0.2 mg/L was most suitable for buds induction and multiplication, where the mul- tiplication was 3.24. The optimal medium for strengthing seedlings and rooting seedlings was 1/2MS＋6-BA 0.6 rag/ L＋NAA 0.5 mg/L＋20% potato juice sucrose. The rooting rate was up to 95%. The seedlings could be used for transplanting from training bottles to the greenhouse when had 3 roots. The garden soil：orchid soil=1： 1 was the best material where the survival rate could be up to 63.64%. Conclusion： The rapid propagation system of can be established by the aseptic tubers with buds, which could promote the artificial cultivation of L.nigra Seidenf.
出处
《生物技术通讯》
CAS
2013年第2期235-237,共3页
Letters in Biotechnology
关键词
紫花羊耳蒜
组织培养
增殖
生根
炼苗
Liparis nigra Seidenf.
tissue culture
multiplication
root
transplantin
