摘要
目的 探讨Ｎ－基－Ｄ－天门冬氨酸（Ｎ-methyl-Ｄ-aspartate,ＮＭＤＡ）和非ＮＭＤＡ类受体在基本呼吸节律发生和调 节中的作用。方法 在新生ＳＤ大鼠离休延髓脑片上记录舌下神经的呼吸节律性放电活动，在改良的Krebs液中加入兴 奋性氨基酸类递质及相应的拮抗剂，观察其ＲＲＤＡ的影响。结果 使用非ＮＭＤＡ受体激动剂海人酸（Kainic acid.ＫＡ） 后，可见呼吸周期及呼气时间有所延长， ＮＭＤＡ受体激动剂对呼吸活动则没有明显影响（Ｐ＞０．１）；相应的拮抗剂６－氰基 －７－硝基喹恶啉土卫四（６，７-dinitroquinoxaline-2,3-dione,ＤＮＱＸ）和２－氨基磷酸戊酸（D-２-amino-5-phosphonopentanoic, ＡＰ5）均可使放电频率和积分幅值明显降低吸气时间显著缩短（Ｐ＜０.０１），ＤＮＱＸ同时可致呼吸周期和呼气时间明显缩短 （Ｐ＜０．０５）。结论 在哺乳动物基本呼吸节律的产生和调节中，ＮＭＤＡ类受体主要对呼吸活动的强度产生调节作用；而非 ＮＭＤＡ类受体不仅可以影响呼吸的强度，同时对呼吸的频率也发挥调节作用。
Objective To study the role of N-methyl-D-aspartate (NMDA) and non-NMDA receptors in the generation and modulation of basic respiratory rhythm. Methods Respiratory rhythmical discharge activity (RRDA) of the hypoglossal nerve was recorded by suction electrode on the brainstem slices isolated from the neonatal rats, and the effects of the excitatory amino acids and its antagonists on the RRDA were investigated by adding these drugs into the modified Kreb's solution perfusing the brainstem slices. Results After application of the non-NMDA receptors agonist KA, it was found that the respiratory cycle and the expiratory time were slightly lengthened, but the NMDA receptor agonist NMDA had no effect on the RRDA. Both of the mutual antagonist DNQX and AP5 remarkably decreased the discharge frequency and the integral amplitude, accompanied by the shortening of the inspiratory time; DNQA simultaneously shortened the respiratory cycle and the expiratory time. Conclusion During the generation and the modulation of the mammalian respiratory rhythm, NMDA receptors act mainly to regulate the amplitude of the respiratory activity, and the non-NMDA receptors can not only affect the respiratory amplitude but also modulate the respiratory rhythm.
出处
《第一军医大学学报》
CSCD
北大核心
2001年第4期251-254,共4页
Journal of First Military Medical University
基金
国家自然科学基金!(39670278)
