摘要
目的研究苯巴比妥钠对新生大鼠节律性呼吸产生及其调节的影响。方法制作新生大鼠离体延髓脑片标本,同时保留舌下神经根,给予灌流改良Krebs液,记录舌下神经根的呼吸相关节律性放电活动(RRDA)及吸气神经元单位的放电活动。在改良Krebs液中分别单独加入巴比妥类药物苯巴比妥钠,终浓度分别为20、40、60、80μmol/L,持续灌流1h,观察舌下神经根RRDA及吸气神经元单位电活动的变化。加入荷包牡丹碱,进一步探讨苯巴比妥钠影响呼吸的可能机制。结果20、40、60μmol/L组间RRDA变化差异显著,而且呈浓度依赖性变化,60和80μmol/L组间舌下神经根RRDA的吸气时程、整合放电积分幅度的变化无显著差异。荷包牡丹碱能够部分恢复苯巴比妥钠所导致的RRDA的抑制。结论苯巴比妥钠能够降低新生大鼠离体脑片舌下神经根RRDA,且GABAA受体参与是介导苯巴比妥钠抑制延髓脑片呼吸的机制之一。
Objective To study the effects of pentobarbital sodium in generation and modulation of rhythmical respiration in neonatal rats. Methods The effects of pentobarbital sodium were examined on hypoglossal nerve （Ⅻ） rootlets and inspiratory neurons in the medullary preparations including the medial region of the nucleus retrofacialis, pre-Bǒtzinger complex and the dorsal respiratory group of neonatal rats aged 0-3 days. The electrical activity of Ⅻ nerve rootlets and inspiratory neurons were recorded. Different doses of pentobarbital sodium （20, 40, 60, 80 μmol/L） were added into modified Krebs solution to observe changes in the discharge activity of Ⅻ nerve and inspiratory neurons. Bicuculline was used to further investigate the mechanisms that pentobarbital sodium suppresses respiration. Results The discharge activity inhibition of Ⅻ nerve was increased as pentobarbital sodium doses increased from 20 to 60 μmol/L, but no significant difference was observed between the doses of 60 and 80 μmol/L. Bicuculline can partly restore the rhythmical respiration discharge activity. Conclusion Pentobarbital sodium can suppress respiration partly via GABAA receptors.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2006年第9期1273-1275,1279,共4页
Journal of Southern Medical University
基金
国家自然科学基金(30570670)
广东省自然科学基金(5004714)~~
关键词
苯巴比妥钠
呼吸节律
GABAA受体
面神经后核内侧区
延髓脑片
pentobarbital sodium
respiratory rhythms
GABAA receptors
medial region, nucleus retrofacialis
medullary preparation
