摘要
采用本实验室构建的丝状蓝藻Calothrixsp.PCC7601基因整合平台系统供体质粒pUTK转化单胞蓝藻Synechococcussp.PCC7942,通过抗性筛选获得了具卡那霉素抗性的转化藻株。经Southern杂交证实 pUTK部分序列已整合到Synechococcussp.7942染色体DNA上 ;红光诱导后通过蛋白质SDS PAGE电泳 ,Westernblot证实 pUTK的胸腺素α1 基因得以有效表达,表达量达到总蛋白的4.8 %。结果表明,基因整合平台系统供体质粒 pUTK不仅可转化丝状蓝藻Calothrixsp.PCC7601,还可转化单胞蓝藻Synechococcussp.PCC7942。
Donor plasmid pUTK constructed for transferring Calothrix sp.PCC7601 was introduced into Synechococcus sp. PCC7942 by natural transformation. Screened by kanamycin and detected by Southern blot analysis, the transformants which occurred by plasmid homology integration were obtained. Thymosin α1 was expressed up to 4.8% of total protein mass by red light inducing, which was tested by SDS PAGE and confirmed by Western blot. The results showed that the donor plasmid pUTK can be used to transfer not only filament blue green algae Calothrix sp.PCC7601, but also single cell blue green algae Synechococcus sp.PCC7942?
出处
《海洋科学》
CAS
CSCD
北大核心
2001年第6期1-3,34,共4页
Marine Sciences
基金
国家863计划资助项目819 04 03号
