摘要
研制了适合纯化猪血超氧物岐化酶(Cu.Zn-SOD)溶液的醋酸纤维素(CA)超滤膜,其截留分子量约为16 000。研究了溶液浓度、操作压力等因素对通量的影响。结果表明,自制CA膜超滤纯化SOD溶液性能优于一些市售膜。收率为86～89%,比活提高25%,纯化因子1.35。利用透析过滤法,基本上能除去溶液中游离铜离子。
A cellulose acetate ultrafiltration membrane with molecular weight cut-off of 16000 was prepared and tested for the purification of pig blood superoxide dismutase (Cu. Zn-SOD) from its solution. The effects of the concentration and the operation pressure on flux were investigated. With a yield of 86～89%, a raise of specific activity of SOD by 25% and a purification rate of 1.35, the results showed that the CA ultrafiltration membrane was better than commerical ones. The free cupric ions in the solution were basically removed by diafiltration,
关键词
歧化酶
提纯
过滤膜
superoxide dismutase
ultrafiltration membrane
cellulose acetate
diafiltration
