摘要
用免疫组化LSAB法检测人乳腺癌SK BR 3细胞表皮生长因子受体 (EGFR)的表达 ,用结晶紫染色法和3 H 亮氨酸掺入法检测TP40对SK BR 3细胞生长及蛋白质合成的抑制 ,用过量EGF竞争拮抗TP40的细胞毒作用。结果显示 :SK BR 3细胞免疫组化显示出大量棕黄色的EGFR阳性染色。TP40浓度为 1～ 10 0 μg/L时 ,对SK BR 3细胞生长及蛋白质合成呈剂量依赖性抑制。过量EGF能完全拮抗TP40的细胞毒作用。提示 :人乳腺癌SK BR 3细胞能高水平地表达EGFR ,重组TP40对SK BR 3细胞生长具有较强的抑制作用 ,作用部位为细胞的EGFR。
Expression of epidermal growth factor receptor (EGFR) in SK BR 3 cells, a human breast carcinoma cell line,was analysed with immunohistochemistry. Inhibitory effects of TP40 on SK BR 3 cells growth and protein synthesis were analysed with crystal violet staining and 3 H leucine incorporation. Competitive assays were performed by the addition of excess of EGF. The results showed that the SK BR 3 cells exhibited large amounts of brown immunoperoxidase reaction indicative of EGFR. When the concentration of TP40 was in the range of 1 100μg/L,TP40 inhibited SK BR 3 cell growth and protein synthsis in a dose dependent form. An excess of EGF could completely block inhibitory effects of TP40. The results suggested that the human breast carcinoma SK BR 3 cells express EGFR at a high level. TP40 could significantly inhibit the growth of SK BR 3 cells. The cytotoxic effects of TP40 were specifically mediated by EGFR.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2001年第5期317-318,F004,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金!(批准号 3 9670 3 16)资助
