摘要
目的 通过exvivo途径 ,利用基因工程化细胞对酪氨酸羟化酶 (TH)基因在治疗帕金森病中的作用进行探讨。方法 将TH基因构建入逆转录病毒载体中 ,再将含重组DNA的病毒上清感染培养的星形胶质细胞移植入PD大鼠脑内 ,了解PD鼠旋转行为的改善情况。结果 TH基因治疗组PD鼠旋转行为改善明显。结论 TH对PD有明显的治疗作用。
Objective To explore the role of tyrosine hydroxylase (TH) gene in the treatment of Parkinson's disease (PD) by using ex vivo gene transfer. Methods After the construction of TH gene in a retroviral vector, the astrocytes were cultured with the supernatant containing the recombinant DNA and then grafted into the cerebrum of PD rats. The reduction of the rat rotation beharior was evaluated. Results The rotation of PD rats was markedly improved in the rats with ex vivo gene transfer. Conclusion TH has an obvious efficiency on the treatment of PD and the astrocytes can be used as effective gene transfer cells.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2001年第5期556-558,共3页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目! ( 39370 686)
