摘要
目的观察脑源性神经营养因子（ＢＤＮＦ）基因工程成肌细胞脑内纹状体移植对帕金森病大鼠的治疗作用。方法建立逆转录病毒介导的ＢＤＮＦ表达质粒并转染成肌细胞，筛选阳性细胞进行脑内移植。结果基因工程成肌细胞脑内移植可明显提高帕金森病大鼠黑质酪氨酸羟化酶（ＴＨ）阳性神经元的存活率，使纹状体多巴胺含量明显增加，动物的旋转行为改善约５０％，并持续２个月之久。结论脑源性神经营养因子基因工程成肌细胞脑内纹状体移植可明显改善动物的旋转行为并可促进黑质神经原的存活。
Objective To observe the role of genetically engineered myoblast in the treatment of rat Parkinson disease models Methods Genetically modified myoblasts to produce brain derived neurotrophic factors (BDNF) were established and grafted into the corpora striata of Parkinson disease modelsin rats. The behavior and pathological changes were observed and the Dopamine contents in the corbora striata were measured with high performance liquid chromatography (HPLC). Results The intracranial graft of genetically engineered myoblasts can promote the survival of TH positive neurons in the nigra of Parkinson's rat and the content of Dopamine in the striatum were increased significantly. The rotation behavior improved 50% after the transplantation. Conclusion It is suggested that BDNF secreting myoblasts can promote the survival of the neuron and provide a new and effective method for the treatment of Parkinson disease.
出处
《中华神经科杂志》
CSCD
1999年第1期13-15,共3页
Chinese Journal of Neurology
关键词
脑源性
神经生长因子
基因疗法
震颤性麻痹
Brain derived neurotrophic factor
Parkinson disease
Gene therapy
