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筛选阿昔洛韦对角朊细胞的最大效应量

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摘要 目的 利用MTT方法和细胞直接记数法筛选阿昔洛韦对正常角朊细胞的最大效应量。方法 将阿昔洛韦加到体外培养的正常人角朊细胞中,3天后加入MTT,在550nm测其吸光度值,同时将细胞消化在显微镜下计数细胞数量。结果 两种方法测得阿昔洛韦在300μg/mL时出现了对正常角朊细胞的细胞毒作用,其最大效应量为200μg/mL。结论 该研究为今后进行阿昔洛韦的体外实验提供依据。
出处 《岭南皮肤性病科杂志》 2000年第1期1-4,共4页 Southern China Journal of Dermato-Venereology
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