摘要
用体外培养人角朊细胞研究了１．２５－双羟维生素Ｄ_３（１，２５（ＯＨ）_２Ｄ_３）对角朊细胞增殖，细胞形态及ＨＬＡ－ＤＲ，ＩＣＡＭ－１分子表达的影响，培养液中加入１，２５（ＯＨ）_２Ｄ_３可使细胞增殖明显抑制。用流式细胞仪测定，１，２５（ＯＨ）_２Ｄ_３处理６天后角朊细胞体积较未处理的明显变大。用免疫组化染色显示１，２５（ＯＨ）_２Ｄ_３对经ＩＦＮ－γ和ＴＮＦａ刺激的角朊细胞ＨＬＡ－ＤＲ和ＩＣＡＭ－１分子表达无明显影响。
The effects of 1,25 -dihydroxyvitamin D_3(1,25(OH)_2D_3)on cell proliferation,cell morphological alteration and expression of HLA-DR and ICAM-1 molecules in cultured human keratinocytes (NHKs)were studied,NHK proliferation was significantly inhibited by adding of 1,25(OH)_2D_3 into culture media. Flowcytometry assay revealed that 1,25(OH)_2D_3 treatment resulted in a enlargement of the size of cultured NHKs,which was also associated with a inhibition in cell proliferation .The immunohistological technque by interferon-γ and tumor necrosis factor-α.
出处
《临床皮肤科杂志》
CSCD
北大核心
1995年第6期342-344,共3页
Journal of Clinical Dermatology
