摘要
目的 :在离体条件下 ,通过猪脑微管蛋白的聚合和解聚合反应 ,分析两种乙酰胆碱受体抑制剂—阿托品和筒箭毒碱对微管蛋白聚合的影响。方法 :将受试物加入微管蛋白聚合 /解聚合反应体系中 ,于 37℃、35 0nm下检测反应体系吸光值的变化。结果 :阿托品和筒箭毒碱均使反应体系吸光值显著下降 ,提示它们对微管蛋白聚合具有明显的抑制效应。结论 :受试化合物可能通过影响微管蛋白聚合来影响有丝分裂进而引发非整倍体。
Purpose: Two kinds of antagonists to cholinergic receptors, atropine sulfate and d tubocurarine, were analyzed for their effects on porcine tubulin polymerization. Methods: Two chemicals were added into the tubulin polymerization/depolymerization system and the changes of absorption values at value decreased significantly at the reaction solution. The results indicated that 350 nm and 37℃ were recorded. Results: The chemicalsmade the absorption value decreased significantly at the reaction solution. The results indicated that the chemicals strongly inhibit the polymerization of tubulin. Conclusion: The tested chemicals interrupt the mitosis by tubulin polymerization and may induce aneuploidy by the pathway.
出处
《癌变·畸变·突变》
CAS
CSCD
2001年第2期70-73,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金!( 3 9660 0 3 3 )
云南省科委国际合作计划!( 98C0 13 )资助
