摘要
目的 探讨缓激肽 β2 受体启动子基因 5 8T/C多态性与中国汉族人群的高血压病相关性。方法 运用多酶链式反应、启动子区单链构象多态性和基因克隆、测序等方法了解 10 6例高血压病患者和性别、年龄相匹配的 98名健康人中 5 8T/C基因分布情况。结果 等位基因C、T在高血压病患者和健康人的分布频率分别为 0 .5 8、0 .4 2和 0 .4 6、0 .5 4 ,基因分布频率符合Hardy Weinberg平衡。两组人群的基因型和等位基因分布频率存在明显统计学差异 (P =0 .0 3 2、P =0 .0 18)。结论 人类缓激肽 β2 受体启动子基因 5 8T/C突变可能是中国汉族人群发生高血压病的独立危险因素。
Objective The present study examined the genetic contribution of the human β 2 bradykinin receptor gene to essential hypertension and identified the association of a -58T/C polymorphism with essential hypertension in a Chinese Han population. Methods The study consisted of 106 hypertensive subjects and 98 age-and sex-matched controls. The distribution of -58T/C polymorphism was measured in patients and controls by using PCR, SSCP, cloning and sequencing. Results The allelic frequencies were 0.58 for the C allele in 0.42 for the T allele in hypertensive subjects, and 0.46 for the C allele and 0.54 for the T allele in normotensive subjects. The allelic frequencies were in Hardy-Weinberg equilibrium. Significant differences in the genotype distribution (P=0.032) and allelic frequencies (P=0.018) between hypertensive and normotensive subjects were seen. Conclusion These results suggest that -58C allele of the human β 2 bradykinin receptor gene may be an independent risk factor for essential hypertension in the Chinese Han population.
出处
《中华心血管病杂志》
CAS
CSCD
北大核心
2001年第4期203-205,共3页
Chinese Journal of Cardiology
