摘要
通过电镜观察，应用形态计量学的方法计量了氟中毒组与对照组大鼠肝细胞线粒体的体密度（Ｖｖｎ）、面密度（Ｓｖｎ）、比表面（Ｑｍ）、面数密度（Ｎａｍ）、规化形状因子（ＲＦＦｍ）；肝细胞粗面内质网的体密度（Ｖｖｒ）、面密度（Ｓｖｒ）；肝细胞核的体密度（Ｖｖｎ）、面密度（Ｓｖｎ）。结果表明：染氟组大鼠（１）肝细胞线粒体的ＲＦＦｍ显著降低（Ｐ＜０．０１），Ｖｖｍ、Ｓｖｍ非常显著增高（Ｐ＜０．００５，Ｐ＜０．０１），Ｑｍ无明显改变，Ｎａｍ呈增高趋势，这提示氟能引起肝细胞线粒体的畸型和增生；（２）肝细胞粗面内质网的Ｖｖｒ、Ｓｖｒ均呈降低趋势，提示氟可引起肝细胞粗面内质网的减少和破坏；（３）肝细胞核的Ｖｖｎ、Ｓｖｎ非常显著和显著增高（Ｐ＜０．０１，Ｐ＜０．０５），提示氟可引起肝细胞活跃增生。
By using morphological quantitative method,the following indicators were measured in liver cells of rats with fluorosis group and control group:Volume density (Vvm),Surface density (Svm),Specific Surface (Qm),Area number density (Nam),Regular form factor (RFFm) in mitochondria;Vvr,Svr in rough surfaced endoplasmic reticulum;Vvn,Svn in nuclei.The results showed RFFm,Vvr and Svr decreased markedly,Vvm,Svm,Vvn and Svn increased significantly,but Qm and Nam had no change in fluorosis group as compared with the control.These resuts suggested that F- could induce proliferation in liver cells,deformation in mitochondria,and decrement and destraction in rough surfaced endoplasmic reticulum.
出处
《中国地方病学杂志》
CAS
CSCD
1998年第2期98-100,共3页
Chinese Jouranl of Endemiology
