摘要
在Tris缓冲溶液中 ,核酸能与某些碱性三苯甲烷染料如乙基紫 (EthylViolet,EV)、结晶紫 (CrystalViolet,CV)和甲基紫 (MethylViolet,MV)结合而使共振瑞利散射 (RRS)急剧增强并产生新的RRS光谱 .以鲱鱼精DNA (HerringSpermDNA ,hsDNA)为例考察了其相应的光谱特征、影响因素和适宜的反应条件 ,体系均在hsDNA浓度为 0～ 2 0μg/mL时与散射强度呈直线关系 ,方法具有较高的灵敏度 ,其检出限 (3σ)分别为 4 7ng/mL(EV hsDNA体系 ) ,13 0ng/mL (CV hsDNA体系 )和 12 2ng/mL (MV hsDNA体系 ) .考察了某些共存物质的影响 ,并用于合成样品中核酸的测定 。
In a medium of Tris buffer solution, nucleic acids can bind with some basic triphenylmethane dyes including ethyl violet(EV), crystal violet(CV) and methyl violet(MV) to form new products, resulting in the great enhancement of RRS and new RRS spectra. The intensity of RRS is directly proportional to the concentration of hsDNA in the range of 0～2 0 ug/mL for all system. The method has fairly high sensitivity and the detecting limits of hsDNA (3σ) are 4 7ng/mL for EV hsDNA system, 13 ng/mL for CV hsDNA system and 12 2 ng/mL for MV hsDNA system,respectively. The influence of some coexisiting substance was investigated and the method was applied to the determination of trace amounts of nucleic acid in synthetic samples with satisfactory results.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第2期164-169,共6页
Journal of Southwest China Normal University(Natural Science Edition)
基金
国家自然科学基金资助项目 ( 2 9875 0 1P)
