摘要
目的 了解抑肽酶对心瓣膜置换术患者围术期全身炎症应答的影响。 方法 将接受心瓣膜置换术的16例患者随机分为 :对照组 (n=8) ,不用抑肽酶 ;治疗组 (n=8) ,于预冲液中加入抑肽酶 30 0万单位 ,分别于术前、停机、停机后 1小时和术后 1天抽取外周血 2 ml,收集血清用酶联免疫吸附测定 (EL ISA)双抗夹心法检测白细胞介素 - 6(IL- 6 )和白细胞介素 - 8(IL- 8)。 结果 体外循环术后患者血清 IL- 6和 IL- 8水平升高 (P<0 .0 5 ) ;术后 1天仍高于术前 ;停机后 1小时治疗组 IL - 6水平和停机时 IL - 8水平低于对照组 ,但差别均无显著性意义 (P>0 .0 5 )。 结论 尽管抑肽酶有抗炎症效应 ,但仅预冲液中加入抑肽酶 30 0万单位无法有效抑制心瓣膜置换术患者围术期促炎性细胞因子IL - 6和 IL -
Objective To explore the effect of Aprotintin on whole-body inflammatory response in the patients with cardiac valve replacement during perioperative period. Methods Sixteen patients undergoing cardiac valve replacement were randomized to control group (n=8) which received an equivalent volume prime without aprotinin and the aprotinin therapy group (n=8) which received 3×106 IU of aprotinin added to the priming solution of the extracorporeal circulation (ECC). Serum samples were collected before the operation, at the end of ECC, 1 h after the end of ECC and 1 d after the operation respectively. interleukin-6(IL-6) and interleukin-8(IL-8) were assayed by enzyme-linked immunoadsorbent techniques. Results After ECC the levels of IL-6 and IL-8 increased significantly in both groups (P<0.05). The levels of 1d after the operation were still higher than that before the operation in both groups, but there were no statistic significance (P>0.05). At 1h after the end of ECC, the level of IL-6 in aprotinin group was lower than that of control group (P>0.05). At the end of ECC, the level of IL-8 in aprotinin group was lower than that of control group (P>0.05)。 Conclusion Although aprotinin has antiinflammatory activity, but pump prime adding aprotinin 3×106 IU only may fail to attenuate proinflammatory cytokine release (IL-6,IL-8) effectively in patients with cardiac valve replacement during perioperative period.
出处
《中国胸心血管外科临床杂志》
CAS
2001年第2期95-97,共3页
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
基金
四川省科委资助课题!(98-G0 12 8)&&
