摘要
为了更好地了解宿主对禽流感病毒(AIV)感染后的免疫反应,本试验以TC-1细胞为模型,感染H9N2亚型AIV。通过荧光定量PCR(qRT-PCR)和酶联免疫吸附试验(ELISA)方法检测了3种模式识别受体(TLR-3、RIG-I和MDA-5)和5种炎性细胞因子(IL-6、RANTES、IP-10、TNF-α和IFN-β)。结果显示:3种模式识别受体中MDA-5上调最为明显,5种炎性因子中RANTES和IP-10上调最为明显;而IL-6、TNF-α和IFN-β上调相对较平缓。本研究结果表明:在病毒感染过程中MDA-5反应较TLR-3和RIG-I更为强烈,而在炎性反应过程中RANTES和IP-10反应更加剧烈,IL-6、TNF-α和IFN-β反应则相对较温和一些。本研究在一定程度上反映了机体对流感病毒的免疫反应机制,为在生产中疫苗的使用具有一定的指导意义。
In order to know more about the immune response of the host organism to the infection of avian influenza virus（AIV）, TC-1 cells were set as a model, which infected by H9N2 subtype AIV. Three kinds of pattern recognition receptors（TLR-g,RIG-I and MDA-5） and 5 kinds of in- flammatory cytokines（IL-6,RANTES, IP-10, TNF-α and IFN-β） were detected by quantitative re- al-time polymerase chain reaction（qRT-PCR） and enzyme-linked immunosorbent assay（ELISA） methods. The results showed that there was obvious up-regulation in MDA-5 ,RANTES and IP-10 among the 3 kinds of pattern recognition receptors and 5 kinds of inflammatory cytokines, while IL-6, TNF-α and IFN-β were slight increase. The results indicated that MDA-5 played more impor- tant role in the identification of the virus TLR-3 and RIG-I,meanwhile,IP-10 and RANTES were more intense in the inflammatory reaction, and that of IL-6, TNF-α and IFN-β were relatively mod- est. To a certain extent, this study reflects the mechanism of the host immune response to influen- za virus,and it has a certain guiding significance for the use of the vaccine in the production.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2017年第6期1084-1091,共8页
Chinese Journal of Veterinary Science
基金
国家自然科学基金2014年面上资助项目(31470258)
病原微生物生物安全国家重点实验室开放研究基金资助项目(SKLPBS1449)
山东省自然科学基金资助项目(ZR2012CM041)
