摘要
①目的 建立一种简单、灵敏、高效的测定粮食中雪腐镰刀菌烯醇 (NIV )、脱氧雪腐镰刀菌烯醇(DON)、新茄病镰刀菌烯醇 (NS)的方法。②方法 粮食样品用甲醇 水 (95∶5 ,V∶V)提取 ,经Florisil柱净化 ,用 9∶1的氯仿 甲醇进行洗脱 ,提取液经n 7氟丁酰咪唑衍生后 ,用配有SE 5 4弹性石英毛细管柱的气相色谱进行测定 ,检测器为电子捕获检测器。③结果 建立了粮食中 3种镰刀菌毒素的气相色谱测定方法 ,其最低检测限为 1×10 -11～ 1× 10 -10 g ,各毒素回收率均在 85 %以上。④结论 该方法灵敏、简单、快速、回收率高 ,具有很好的实际应用价值。
Objective\ To set up a simple method for detecting three kinds of fusarium mycotoxin in grains. \ Methods Mycotoxins were extracted with methanol water(95∶5, V∶V ) and purified by chomatographic procedure using Florisil column. The column filters were concentrated and the residue was dissolved in methanol. A portion of the solution was reacted with n heptaflurobylimidazol and analysed with gas chromatography. \ Results\ The limit for detection of the three mycotoxins was 1× 10 -11 -1×10 -10 g, and the recoveries were all over 85%. \ Conclusion\ This method was simple and highly efficient. [
出处
《青岛大学医学院学报》
CAS
2001年第1期35-36,共2页
Acta Academiae Medicinae Qingdao Universitatis
