摘要
应用离体细胞培养 ,研究了反义 c-ras寡脱氧核苷酸 (反义 c-ras ODN)对人绒毛膜促性腺激素 (h CG)诱导的大鼠颗粒细胞和黄体细胞生成孕酮 (P)的影响及其与外源性 c AMP和 Ca2 +的关系。结果发现 ,2 0 μmol/ L 反义 c-ras ODN能显著抑制 h CG诱导颗粒细胞的 P生成量 (P<0 .0 5 ) ,而对黄体细胞的 P生成无明显影响 ;反义 c-ras ODN对h CG诱导的颗粒细胞生成 P的抑制作用能被加入 1 0 - 4mol/ L 二丁酰 c AMP所逆转 ,钙通道阻断剂维拉帕米对抑制作用具有协同效应。结果提示 ,c-ras癌基因参与 h CG诱导的颗粒细胞生成 P的调控 ,而对 h CG诱导的黄体细胞
Objective: The effect of c ras antisence oligodeoxynucleotide (antisence c ras ODN) on human chorionic gonadotropin (hCG) induced progesterone production in isolated rat granulosa cells and luteal cells in relation to cAMP and Ca 2+ were investigated. Design: Laboratory studies. Setting :Laboratory in Department of Physiology, Jiangxi Medical College. Method: Incubation of isolated rat granulosa cells and luteal cells in vitro. Results: 20 μmol/L antisense c ras ODN inhibited significantly hCG induced progesterone production in cultured rat granulosa cells ( P <0 05),but no similar effect on luteal cells was observed. Further experiments showed that the inhibition of antisence c ras ODN on the hCG induced progesterone production of rat granulosa cells could be reversed by addition of dbcAMP (10 -4 mol/L), while verapamil (10 -6 mol/L) had a synergistic effect on the inhibitory action. Conclusion: c ras may play an important role in the progesterone production in cultured granulosa cells, but not in luteal cells.
出处
《生殖医学杂志》
CAS
2000年第2期96-99,共4页
Journal of Reproductive Medicine
