摘要
目的探讨中药蜈蚣、紫草、莪术、水蛭的乙醇提取物对大鼠卵巢妊娠黄体细胞分泌孕酮(P)与雌二醇(E2)的影响。方法在大鼠妊娠第4天取卵巢,收集黄体细胞体外培养2 h后,分别加入3个浓度(0.01、0.1及1 mg/ml)的蜈蚣、紫草、莪术、水蛭乙醇提取物,乙醇的总浓度<5%;对照则代之以5%的乙醇生理盐水。在药物作用4 h后,收集部分上清,采用放射免疫法测定P与E2的含量,继续培养4 h,用MTT比色法反映活细胞数量。结果在四种中药作用下,各浓度的吸光度值均有增加。其中莪术、水蛭、蜈蚣均有增加黄体细胞分泌的趋势,尤以水蛭刺激黄体细胞分泌E2的作用最为显著,3个浓度与对照比较差异均有显著性(P<0.01,P<0.05,P<0.01),E2/P比值显著高于对照组(P<0.01,P<0.05,P<0.001);而紫草则有降低黄体细胞分泌E2的作用,随着浓度的降低,抑制作用逐渐增强(P>0.05,P<0.05,P<0.01)。结论提示莪术、水蛭和蜈蚣刺激黄体细胞分泌E2与P,增加E2/P比值,紫草则抑制黄体细胞分泌E2与P,降低E2/P比值,其中紫草与水蛭的作用较强,主要表现在对E2分泌的影响。
Objective： To investigate the effects of Chinese traditional medicine on the estradiol （E2） and progesterone （P） secretion by rat pregnant luteal cells. Methods. Rat luteal cells were collected on day 4 of pregnancy and cultured in M199 medium supplemented with 5% FBS at 37℃ in a humidified atmosphere with 5% CO2. After 2-hour pre-incubation, 4 kinds of Chinese traditional medicine （Wugong, Zicao, Ezhu and Shuizhi）-ethanol extracts were added into the media respectively at three concentrations （0.01 mg/ml, 0.1 mg/ml, 1mg/ml）. The media were collected 4 hours later for the determination of estradiol and progesterone levels by radioimmunoassay, and the living cell quantity was evaluated by MTT assay. Results： All of the 4 kinds of Chinese traditional medicine-ethanol extracts increased the quantity of living luteal cells with different degrees. Wugong, Ezhu and Shuizhi tended to stimulate the hormone secretion of cultured cells, and the significant effect was observed in the Shuizhi group. Shuizhi significantly increased the E2 production of the cultured cells at three concentrations （P〈0.01, P〈0.05 and P〈 0.01, respectively）, which gives a higher E2/P ratio（P〈0. 01, P〈0.05 and P〈0. 001 respectively）. Zicao, however, inhibited the E2 and P production, especially the E2 production by the luteal cells. Conclusions： Ezhu, Wugong and Shuizhi could stimulate the secretion of E2 and P by the cultured luteal cells, and give a higher E2/P value, while Zicao could depress the E2 and P secretion and give a lower E2/P value. Wugong and Zicao have stronger effects on the E2 secretion by the luteal cells.
出处
《生殖医学杂志》
CAS
2006年第2期114-117,共4页
Journal of Reproductive Medicine
基金
北京市教委科技发展计划项目(1999-2002)
关键词
中药
黄体细胞
孕酮
雌二醇
大鼠
Chinese traditional medicine
Progesterone
Estradiol
Rat
