摘要
[目的 ]了解携 L EE毒力岛大肠埃希菌在杭州不同人群中的流行状况。 [方法 ]应用 PCR技术检测不同临床来源的大肠埃希菌菌株的 L EE毒力岛 ,并对检出的 intimin基因 3'端部分以 PCR法和限制性酶切分析法进一步分型。[结果 ]在杭州市区 2周岁以下的腹泻婴幼儿中 ,携 L EE毒力岛的大肠埃希菌的检出率高达 2 0 .0 % ,而在 2岁以上的腹泻人群中检出率仅为 3.0 %。检获的大肠埃希菌 L EE毒力岛的 intimin基因以 β型为主 (占 45 .0 % ) ,并有 2 0 .0 %不能按本文 PCR方法分型。限制酶切分析表明 ,β型可进一步分为 2个亚型 ,本文分离菌株的 γ型 intimin基因与 EHEC O15 7∶H7933株的 γ型 intimin基因酶切图谱差异较大。检获的携 L EE毒力岛的大肠埃希菌菌株中 ,仅有 1/ 5能用国内目前的致泻性大肠埃希菌诊断血清确定 O抗原。 [结论 ]携 L EE毒力岛大肠埃希菌为杭州市致婴幼儿腹泻的主要细菌性病原。L EE毒力岛的检测应成为 EPEC的诊断和流行病学调查主要手段。
Objective] To understand prevalence situation of LEE pathogenicity island positive Escherichia coli strains isolated from Hangzhou. [Methods] LEE (locus of enterocyte effacement) pathogenicity island was determined by PCR in Escherichia coli strains isolated from diarrhea patients, parenteral infection patients and normal persons in Hangzhou. Moreover, the intimin genes found in this paper were typed by PCR and restriction endonuclease analysis according to their 3' end sequences. [Results] The prevalence rates of LEE positive Escherichia coli strains in diarrhea infants and diarrhea patients elder than 3 years old were 20 0 % and 3 04 % respectively. Intimin β gene was found dominantly in LEE positive Escherichia coli strains (45 0 %), and about 20 0 % of the intimin genes were not able to be typed by the PCR method. Restriction endonuclease analysis showed that the intimin β genes could be typed into two subtypes further and the intimin γ genes found this paper were different from intimin γ gene of EHEC O157∶H7 strain EDL933. O serotypes could be determined in only 20% of the LEE positive Escherichia coli strains by diarrheagenic Escherichia coli antisera purchased in domestic market. [Conclusions] LEE positive Escherichia coli may be one of important bacterial pathogens that cause infant diarrhea in Hangzhou. Detection of LEE should be main method for EPEC diagnosis and epidemiological investigation.
出处
《海峡预防医学杂志》
CAS
2001年第1期1-6,共6页
Strait Journal of Preventive Medicine
关键词
大肠埃希菌
LEE毒力岛
流行病学
婴幼儿腹泻
Escherichia coli
locus of enterocyte effacement pathogenicity island
epidemiology
infant diarrhea
