摘要
用碱性磷酸酶标记的ｅａｅＡ基因寡核苷酸探针，对３７个血清群的２２１株从肯尼亚５岁以下腹泻患儿粪便中分离的大肠杆菌进行检测，并对ｅａｅＡ基因阳性菌株进一步做了ｂｆｐＡ和ｓｌｔ基因检测，ＨＥｐ－２细胞粘附及荧光肌纤蛋白染色（ＦＡＳ）试验。结果表明，ｅａｅＡ基因的携带率为１９．５％，ＥＰＥＣ、ＥＨＥＣ和其它血清群中ｅａｅＡ基因的携带率分别为３１．６％、６６．７％和８．９％，根据ｂｆｐＡ和ｓｌｔ基因检测及ＨＥｐ－２细胞粘附和ＦＡＳ试验的结果，可将携带ｅａｅＡ基因的大肠杆菌分成５种类型。提示ｅａｅＡ基因的分布不仅限于ＥＰＥＣ和ＥＨＥＣ，而且携带ｅａｅＡ基因菌株含有多种毒力决定因子。
Two hundred and twenty one strains of Escherichia coli of 37 serogroups isolated from children with diarrhoea in Kenya were examined for the eaeA gene,which encodes the expression of the attaching and effacing lesions of Escherichia coli ,by using an alkaline phospbatase conjugated oligonucleotide probe.The strains positive for eaeA gene were further assayed for bfpA and slt genes,and also for HEp 2 adhesion and fluorescent actin staining (FAS) tests.The results showed that the eaeA prevalence rate was 19 5%,with those in EPEC,EHEC and other serogroups being 31 6%,66 7% and 8 9%,respectively.Based on theprobes for bfpA and slt genes,HEp 2 adhesion and FAS tests,5 virulence patterns were differenciated among eaeA harbouring E.coli .This indicates that eaeA gene is not limited to EPEC and EHEC, that the eaeA harbouring E.coli are heterogeneous with respect to their virulence determinants.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1997年第4期305-308,共4页
Chinese Journal of Microbiology and Immunology
