摘要
目的：探讨血管紧张素 Ⅱ（ＡｕｇⅡ）、环磷酸腺苷（ｃＡＭＰ）对 ＡｎｇⅡ１型（ＡＴ１）受体信使核糖核酸（ｍＲＮＡ）表达的稳定性及心肌细胞蛋白代谢的影响。 方法：３天以内 ＳＤ乳鼠原代培养的心肌细胞，分为对照组（不加刺激因子）、Ａ组（加入放线菌素 Ｄ ５μｇ／ｍｌ）、Ｂ组（加人Ｆｏｒｓｋｏｌｉｎ及ＩＢＭＸ各３０μｍｏｌ／Ｌ）；Ｃ组（加入放线菌素Ｄ ５ μｇ／ｍｌ、Ｆｏｒｓｋｏｌｉｎ及ＩＢＭＸ各３０ μｍｏｌ／Ｌ），与刺激因子作用后，提取细胞ＲＮＡ，逆转录多聚酶链反应测定ＡＴ１受体ｍＲＮＡ含量。 结果：ＡＴ１受体ｍＲＮＡ半衰期约在 ４．９ 小时，ＡｕｇⅡ、放线菌素Ｄ单独或共同作用心肌细胞 ６ 小时ＡＴ１受体ｍＲＮＡ的表达无显著性差异（Ｐ＞０．０５）。Ｂ组ＡＴ１受体ｍＲＮＡ的表达与对照组比较具极显著性差异（Ｐ＜０．０１）。ＡｎｇⅡ、Ｆｏｒｓｋｏｌｉｎ＋ＩＢＭＸ分别或共同作用心肌细胞 １４４小时，使其蛋白含量分别为对照的 １３３． ３％（与对照比有显著性差异，Ｐ＜０．０５）、９２．８％（与对照比无显著性差异，Ｐ＞０．０５）和１０５．０％（与对照比无显著性差异，Ｐ＞０．０５）。 结论：ＡｎｇⅡ不改变ＡＴ１受体ｍＲＮＡ的稳定性，ｃＡＭＰ?
Objective: To study the effect of angiotensin Ⅱ and cAMP on the stability of angiotensin Ⅱ type １receptor messenger ribonucleic acid (ATlmRNA) expression and protein metablism of cells. Methods: Primary cultured cardiomyocytes taken from 1～3d neonatal SD rat hearts were devided into 4 groups: Control group,group A(5 μg/ml actinomycin D),group B(30 μmol/L Forskolin+30 μmol/L IBMX),group C (5 μg/ml actinomycin D+30 μmol/L Forskolin+30 μmol/L IBMX). After Aug Ⅱ treated with different stimulators,the amount of AT1 receptor mRNA of the cells was detected by reverse transcription and polymerase chain reaction(RT-PCR). Results: The half-life of AT1 receptor mRNA is about 4.9h. Cardiomyocytes were treated with Aug Ⅱ or actinomycin D alone or together for 6 h,AT１receptor mRNA levels no significant difference was found among them(p> 0. 05 ). AT１receptor mRNA levers in group B were significant higher than control group (p < 0. 01). After the cardiomyomycytes treated with Aug Ⅱ，Forskolin+IBMX alone or together for 144h. This protein contents campaign with control group were 133. 3% (p<0. 05), 92. 8% (p>0. 05),and 105.0% (p>0. 05) respectively. Conclusions: Aug Ⅱ does not change the stability of AT１receptor mRNA. cAMP may inhibit the increase of cardiomyocyte protein content induced by a long-term Aug Ⅱ stimulation.
出处
《中国循环杂志》
CSCD
北大核心
2001年第2期109-111,共3页
Chinese Circulation Journal
