摘要
本文利用蛋白激酶A(PKA)的激活剂Forsolin对大白鼠胰岛素瘤细胞(RINm5F)上GLP一1受体的基因表达进行了研究。在forskolin的作用下,使类胰高血糖素肽I(GLP—1)受体的转水平明显下降,即GLP一1受体mRNA的量明显减少,出现了GLP一1受体基因表达的负调控,但forskoln可以使GLP—1受体的mRNA的稳定性增加结果表明,GLP—1受体的转录是通过cAMP—蛋白激酶A途径进行调控的,因此,影响此途径的物质对类胰高血糖素肽—1的生理功能以及在临床应用方面起着重要作用。
Glucagon—like peptide(GLP—1)is a potent incretin hormone and mediatesits actions via specific receptors at pancreatic B—cells.The GLP—1 receptor belongsto the seven—transmem- brane receptors coupled to G—proteins.We have presently analyzed the regulation of GLP—1 re- ceptor expression in rat insulinoma—derived B—cells(RINm5F)with forskolin that is activator of protein kinase A(PKA).The GLP—1 receptor mRNA levels were down—regulated during incu- bation of cells with forskolin.But forskolin stabilized the GLP—1 receptor mRNA.The results show that the GLP—1 receptor expression is regulated by cAMP—PKA pathway,thereforthe ma- teries,that influence the pathway,are very important for the functions of GLP—1 and the treat- ment of NIDDM.
关键词
GLP-1
基因表达
FORSKOLIN
蛋白激酶A
Giucagon—like peptide—1(GLP—1)receptor
gene expression
forskolin
cAMP
Protein kinase A(PKA)
