摘要
作者采用免疫组化、免疫荧光染色、流式细胞计数等方法研究脑穿刺损伤灶愈合过程中伤灶组织中大胶质细胞的形态和比例的变化及其规律 ,阐明大胶质细胞在脑损伤后胶质瘢痕增生中的作用。结果发现 ,脑穿刺损伤后 ,大量的胶质原纤维酸性蛋白 (GFAP)免疫组化染色阳性细胞聚集在伤灶周围 ,呈典型的反应性胶质化改变 ;流式细胞计数结果证实 ,GFAP阳性细胞比例显著增高 ,在伤后 2w达高峰 ,为 46 % ;半乳糖脑苷脂 (GC)阳性细胞的形态与比例无明显变化。作者提出 ,星形胶质细胞是胶质瘢痕中增生的主要胶质细胞 ,少突胶质细胞在这个过程中 ,不是一种反应活跃的细胞成分。
The changes of the morphology and percentage of the macroglia surrounding focus of brain stabbing injury (BSI) were observed by immunohistochemistry, immunoinfluorescence stain and flow cytometer(FCM) and the effect of magroglia during glial scar forming were elucidated The results showed that a large number of GFAP immunoreactive positive cells were accumulated around the focus of BSI These cells were hyperplastic, hypertrophic, and emerged swollen cytoplasmic processes The most marked changes were observed at 1 2 week after BSI The results of FCM showed that the percentage of GFAP positive cells increased gradually and reached to a peak during 1～2 week after BSI The peak ratio of GFAP positive was about 46% However, the changes of morphology and number of GC positive cells were not detected after BSI The authors believed that astrocyte is the main macroglia during glial scar formatting The oligodendrocytes is not an active cell during this course
出处
《基础医学与临床》
CSCD
北大核心
2001年第2期158-162,共5页
Basic and Clinical Medicine
基金
重庆市青年科研基金
全军九五科研基金!(No.96M0 90 )
