摘要
目的 :探讨氯丙烯对神经细胞NO生成系统和抗氧化系统的影响。方法 :用NO、MDA、NOS、SOD、GSH PH检测试剂盒研究了氯丙烯对鸡胚脑神经细胞NO、MDA含量和NOS、SOD、GSH PX活性的影响。结果 :与对照组相比 ,随着氯丙烯浓度的增加 ,NO含量细胞内明显增加 (P <0 .0 1) ,细胞外无显著差异 ;NOS活性细胞内中、高剂量组显著增加 (P <0 .0 5,P <0 .0 1) ,细胞外活性仅高剂量组显著增加 (P<0 .0 1) ;细胞MDA含量明显降低 ,并呈明显的剂量 效应关系 (r =- 0 .96 93,P <0 .0 5) ;GSH PX活性细胞内逐渐升高 ,呈明显的剂量 效应关系 (r =0 .9872 ,P <0 .0 5) ,细胞外仅高剂量组明显升高 (P <0 .0 1) ;细胞内T SOD和CuZn SOD活性在 3个剂量均明显下降 (P <0 .0 1)。结论 :氯丙烯可影响NO生成系统和细胞内抗氧化系统 ,这可能是氯丙烯引起细胞内Ca2 + 稳态失调的原因之一。
Objective:To research the poisioning mechanisms of allyl chloride and probe into its effects on NO sythesis system and antioxidation system in chicken embryo nerve cells.Methods:The contents of NO and MDA and the activities of NOS,SOD,GSH PX were done with related kits.Results:With allyl chloride increasing,the content of NO increased signifcantly in cells(P<0.01),but it appeared no different in medium.The activities of NOS increased significantly (P<0.01)both in nerve cells and medium.Cell MDA contents decreased significantly (P< 0.01 ) but GSH PX activity increased significantly both in nerve cells and in medium,(P< 0.01 ).T SOD and CuZn SOD activities decreased markedly (P<0.01).Conclusion:Allyl chloride can disturb NO synthesis and antioxidation in nerve cells.It might be one of the mechanisms of calcium unhomeostasis induced by allyl chloride.
出处
《山东医科大学学报》
2001年第2期176-178,共3页
Acta Academiae Medicinae Shandong
基金
山东省自然科学基金资助课题 (Y97C13 0 49)
