摘要
目的 探讨γ 干扰素 (IFN γ)基因修饰的肝细胞 ,经脾内移植后 ,对小鼠移植性肝肿瘤的治疗作用。方法 用IFN γ重组腺病毒 ,将鼠IFN γ基因转染小鼠BNL·CL2肝细胞 ,同时设转染LacZ基因的病毒对照组和未经基因转染的细胞对照组。 6 0只小鼠 ,脾内注射结肠腺癌细胞株C2 6 ,1周后作肝细胞脾内移植 ,分别设治疗组 (BNL·IFN γ) ,病毒对照组 (BNL·LacZ)和细胞对照组 (BNL·CL2 ) ,2周后检测血清IFN γ、肿瘤坏死因子 α(TNF α)和一氧化氮 (NO)水平、脾脏的细胞毒T淋巴细胞 (CTL)活性及对移植性肝肿瘤的影响 ,并观察荷瘤小鼠的存活期。结果 治疗组与病毒和细胞对照组比较 ,血清IFN γ、TNF α和NO水平明显升高 (P均 <0 .0 1) ,脾脏的CTL活性显著增强 (P <0 .0 1) ,肿瘤体积缩小 ,荷瘤小鼠的存活期明显延长。结论 IFN γ重组腺病毒对移植性肝癌有明显疗效 。
Objective To investigate the therapeutic effects of intrasplenically transplanted interferon γ (IFN γ) gene modified hepatocytes on murine implanted liver carcinoma. Methods Murine fetal hepatocytes (BNL·CL2) were transfected with recombinant adenovirus expressing IFN γ. Two cell lines BNL·Lac Z and BNL·CL2 were taken as control. One week after intrasplenically injected C26 cells(colon carcinoma line), sixty tumor bearing syngeneic mice were intrasplenically transplanted IFN γ gene modified hepatocytes and were divided into treated group (BNL·IFN γ) and two control groups (BNL·Lac Z and BNL·CL2), two weeks later, levels of IFN γ, tumor necrosis factor alpha(TNF α) and nitric oxide(NO) in the serum of liver implanted carcinoma bearing mice were assayed, the cytotoxicity of murine splenic cytotoxic T lymphocyte (CTL) was measured. Mophology of hepatic tumors were studied. The therapeutic effects on the mice with the implanted liver carcinoma were also evaluated. Results In treated group (compared with control groups), the levels of IFN γ, TNF α and NO in the serum increased significantly ( P <0.01), the murine splenic CTL activity was markedly augmented ( P <0.01). The tumor volume substantially reduced, and survived for a longer time than untreated mice.Conclusion INF γ recombinant adenovirus could exert potent therapeutic effects and intrasplenic transplantation of adenovirus mediated IFN γ gene modified hepatocytes could be one of the targeting treatments for implanted liver carcinoma.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2001年第2期79-82,共4页
Chinese Journal of Digestion
