摘要
目的 观察高胆固醇血症 (HC)患者血小板L 精氨酸 (L Arg) 一氧化氮 (NO)系统活性的变化。方法 采用比色法检测 2 1例HC患者和 2 6名健康体检者血小板的NO水平 ,采用放射性核素(3H L 精氨酸 )标记法检测血小板一氧化氮合成酶 (NOS)及L Arg转运系统活性。结果 HC患者血小板NO水平为 (2 4.0 6± 3.70 )nmol 10 8血小板 ,明显低于正常人的 (2 8.39± 4.45 )nmol 10 8血小板 (P <0 .0 1) ;HC患者血小板NOS活性为 (1.46± 0 .47)pmol 10 8血小板 ,较正常人的 (1.81± 0 .5 0 )pmol 10 8血小板明显降低 ;HC患者血小板L Arg转运系统的活性亦显著低于正常人 ,其最大转运速率 (Vm)为(4 6 84± 3.39)pmol·(10 7血小板 ) - 1 ·min- 1 ,明显低于正常人的 (5 3.89± 3.45 )pmol·(10 7血小板 ) - 1 ·min- 1 (P <0 .0 5 ) ,而解离常数 (Kd)则无明显改变 (P >0 .0 5 )。结论 HC患者血小板L Arg
Objective To investigate the changes of the platelet L arginine(L Arg)/nitric oxide(NO) pathway in hypercholesterolemia patients. Methods The platelet NO production in 21 hypercholesterolemia(HC) patients and 26 normal individuals was assayed by spectrum, the NOS activity and the transportation of L Arg were determined by 3H labelled L Arg. Results The NO production in hypercholesterolemic platelets [(24.06±3.70)nmol/10 8platelets] was decreased significantly as compared with normal controls[(28.39±4.45)nmol/10 8platelets](P<0.01),and so did the NOS activity [(1.46±0.47)pmol/10 8 platelets] vs (1.81±0.50)pmol/10 8platelets]. The transportation of L Arg by hypercholesterolemia platelets also decreased significantly than that by normal controls. The Vmax in the former[(46 84±3.39)pmol·(10 7platelets) -1 ·min -1 ] was significantly lower than that in the latter[(53.89±3.45)pmol·(10 7platelets) -1 ·min -1 ]. There was no difference between the two groups in K d(P>0.05). Conclusions The activity of the L arg/NO pathway in hypercholesterolemia platelets is decreased significantly as compared with normal controls, implying that it is probably one of the reasons for the hyperactivity of hypercholesterolemia platelets.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2001年第3期132-134,共3页
Chinese Journal of Hematology
基金
广东省重点科技攻关基金资助项目!(99M0 4810G)
卫生部科研基金资助项目!(98 1 0 89)
