摘要
目的:研制一种较为实用的邪毒致肝血瘀阻证的动物模型。方法:将80只1日龄武汉麻鸭,随机分成正常对照组、病理造模组、秋水仙碱防治组、解毒软肝汤小剂量及大剂量防治组。后4组用DHBV阳性血清攻去雏鸭造模,每周1次,剂量0.1ml/只,从第10W起剂量加大为0.2ml/只,且后3组第11d分别用秋水仙碱、解毒软肝汤小剂量及大剂量灌胃,至第112d结束。实验结束时分别检测球结膜微循环,血液流变学指标,肝功能指标,丙二醛(MDA),肝纤维化指标,做肝组织光镜、电镜观察。结果:动物造模组具有明显的球结膜微循环障碍(P<0.01),血液流变学改变(P<0.01),白蛋白(Alb)降低(P<0.05)和G、MDA升高(P<0.01),以及血清PCⅢ、HA、LN和肝组织Hyp含量升高(P<0.01);组织病理可见肝细胞水肿、脂肪变,间质炎细胞浸润、纤维增生和病毒颗粒。解毒软肝汤除能降低肝纤维化指标(P<0.01)和减轻组织病理学改变外,并能降低MDA及血液流变学指标,改善肝功能及球结膜微循环(P<0.01),并存在剂量依赖关系。结论:邪毒(DHBV)连续攻击能够形成鸭肝血瘀阻证模型,其改变与脂质过氧化损害、血液流变学和微循环障碍、肝细胞变性坏死、胶原纤维增生明显相关。
Objective: To make a practical animal model with liver blood stasis caused by pathogenic factor. Methods: 1-day-old Wuhan ducklings were inoculated from shin-vein with serum containing DHBV to establish liver blood stasis animal model and prevented and treated with Jiedu Ruangan Tang. Series of indexes were observed, such as microcirculation of bulbar conjunctiva, blood rheology, liver funetion, hepatic fibrosis, tissue pathology. Results: The animal models had marked microcirculation disturbence of bulbar conjunctiva and changes in blood rheology. And the albumin decreased, globulin, MDA, serum PC Ⅲ , HA, LN and liver tissue Hyp increased. There existed hepatocytes edema, fatty degeneration, leydig's cells infiltration, fiber regeneration and virus granule from optical and electro microsscopic analysis. Jiede Ruangan Tang could decrease MDA, indexes of hepatic fibrosis and blood rheology, release liver tissue pathological changes, and improve liver function, microcirculation of bulbar conjunctiva (P<0. 01). There existed doses dependance. Conelusions: Pathogenic factor continously attaching duck could form duck liver blood stasis, and markedly related to the damage of lipid peroxide, the changes of blood rheology, microcirculation, disturbance, degeneration and neerosis of hepatocytes, regeneration of collagenous fibers.
出处
《中西医结合肝病杂志》
CAS
2001年第2期88-91,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
湖北省自然科学基金课题(No.96J089)
