摘要
目的 探讨多巴胺诱导PC12细胞凋亡的可能机制。 方法 流式细胞仪检测PC12细胞的凋亡率及Bcl 2和Bax蛋白的表达率。 结果 多巴胺诱导PC12细胞凋亡 ,两者间呈明显的量效和时效关系。 0 45mmol/L多巴胺作用 2 4h ,细胞的凋亡率为 5 3 3%± 3 1%。在凋亡过程中 ,Bcl 2蛋白表达显著降低 ,Bax蛋白表达随之增高。诱导型一氧化氮合成酶 (iNOS)抑制剂和半胱氨酸蛋白酶 3(CPP32 )抑制剂对抗多巴胺诱导PC12细胞的凋亡作用与Bcl 2蛋白增加、Bax蛋白降低有关。 结论 Bcl 2和Bax蛋白是多巴胺诱导PC12细胞凋亡的重要调节蛋白 ,iNOS和CPP32在凋亡过程中可能具有重要作用。
Objective To explore the possible mechanism of dopamine induced apoptosis in PC12 cells. Methods Flow cytometric assay was used to quantitate the apoptotic cells and measure the positive rates of Bcl 2 and Bax proteins in PC12 cells. Results Dopamine induced PC12 cell apoptosis in a dosage and time dependent manner. After 24 h treatment with 0 45 mmol/L dopamine, the percentage of apoptotic PC12 cells was 53 30%±3 14%. During the apoptotic process, it showed a decrease of Bcl 2 protein and an increase of Bax protein in PC12 cell. Inhibitors of iNOS (inducible nitric oxide synthase, iNOS) and Caspase 3 (cysteinyl aspantate specific proteinases 3, Caspase 3 or CPP32) blocked PC12 cells apoptosis by increasing expression of Bcl 2 and decreasing expression of Bax. Conclusions Bcl 2 and Bax proteins might be important regulators in dopamine induced apoptosis in PC12 cells. iNOS and CPP32 might play an important role in the apoptotic process.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2001年第2期124-127,共4页
Chinese Journal of Geriatrics
基金
福建省自然科学基金!重点课题资助!项目 (C982 0 0 4)
