摘要
用放射性同位素γ－３２Ｐ－ＡＴＰ标记人工合成的简单重复序列（ＧＡＴＡ）４，与经ＤｒａⅠ酶切的８个番茄品种的ＤＮＡ进行Ｓｏｕｔｈｅｒｎ杂交，得到的ＤＮＡ指纹在品种间差异很大，在品种内单株间无差异。经统计计算，任意两个品种间具有完全相同ＤＮＡ指纹的概率为１．１×１０－４，呈现了高度的品种特异性。
A simple sequence repeat(GATA)4 radiolabelled with -32P-ATP was hybridized with genomic DNA digested with Dra for DNA fingerprinting tomato cultivars.The results showed that the DNA fingerprinting patterns was highly polymorphic between tomato cultivars,and homomorphic in a given cultivar.According to statistic analysis, the probability for any two cultivars having totally same DNA fingerprinting pattern is 1.1 10-4.
出处
《园艺学报》
CAS
CSCD
北大核心
1999年第1期51-53,共3页
Acta Horticulturae Sinica
